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Characterization of a 7 kDa pollen allergen belonging to the gibberellin‐regulated protein family from three Cupressaceae species
- Source :
- Clinical and Experimental Allergy, Clinical and Experimental Allergy, 2020, ⟨10.1111/cea.13675⟩, Clinical and Experimental Allergy, Wiley, 2020, ⟨10.1111/cea.13675⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- Background Severe allergy to fruits mediated by a 7 kDa allergen belonging to the gibberellin-regulated protein (GRP) family is known to be associated with Cupressaceae pollinosis. Objective To identify and characterize Cupressaceae pollen allergens involved in GRP-related fruit allergy. Methods Pru p 7-related proteins from pollen of Cupressus sempervirens, Juniperus ashei and Cryptomeria japonica were identified using a rabbit anti-Pru p 7 antiserum, purified chromatographically and sequenced by mass spectrometry and bioinformatic comparisons. The C sempervirens protein was produced as a recombinant allergen in Pichia pastoris. IgE antibody binding to pollen GRP proteins was analysed in a peach allergic (n = 54) and a cypress pollen allergic (n = 88) patient population from southern France using ImmunoCAP. Results In each of the three Cupressaceae species studied, a 7 kDa pollen protein related to Pru p 7 was identified and found to comprise an amino acid sequence of 63 residues in length, 92%-98% identical to each other and 67%-68% identical to Pru p 7. The C sempervirens, J ashei and C japonica GRP allergens have been officially recognized by the WHO/IUIS Allergen Nomenclature Sub-Committee and named Cup s 7, Jun a 7 and Cry j 7, respectively. Recombinant Cup s 7 showed IgE antibody binding capacity comparable to that of the purified natural allergen. Among 51 peach allergic subjects sensitized to Pru p 7, substantially higher levels of IgE to Cup s 7 than to Pru p 7 were found. Further, the pollen protein was able to completely outcompete IgE binding to Pru p 7, while the reverse competition effect was modest, consistent with primary sensitization by the pollen allergen. Conclusion and clinical relevance Pru p 7-related pollen allergens from three Cupressaceae species have been characterized and may become useful for the identification of pollinosis patients at risk of developing severe fruit allergy.
- Subjects :
- 0301 basic medicine
Male
Cupressaceae
Cryptomeria
medicine.disease_cause
Immunoglobulin E
0302 clinical medicine
Allergen
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Cupressus sempervirens
Immunology and Allergy
Child
ComputingMilieux_MISCELLANEOUS
Plant Proteins
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
biology
food and beverages
Middle Aged
Child, Preschool
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Pollen
Female
Food Hypersensitivity
Adult
Adolescent
Immunology
Pichia pastoris
Microbiology
03 medical and health sciences
Young Adult
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
otorhinolaryngologic diseases
medicine
Humans
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Aged
Prunus persica
Rhinitis, Allergic, Seasonal
Allergens
Antigens, Plant
medicine.disease
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Molecular Weight
030104 developmental biology
030228 respiratory system
biology.protein
Biomarkers
Fruit allergy
Subjects
Details
- Language :
- English
- ISSN :
- 09547894 and 13652222
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Allergy, Clinical and Experimental Allergy, 2020, ⟨10.1111/cea.13675⟩, Clinical and Experimental Allergy, Wiley, 2020, ⟨10.1111/cea.13675⟩
- Accession number :
- edsair.doi.dedup.....4d942201c664d70942d6367d111b0dcf