Back to Search
Start Over
T cell receptor repertoire of CD4+ and CD8+ T cell subsets in the allogeneic bone marrow transplant recipient
- Source :
- Cancer immunology, immunotherapy : CII. 41(2)
- Publication Year :
- 1995
-
Abstract
- Allogeneic bone marrow transplantation (BMT) has become a therapy of choice for the treatment of certain malignancies and hematopoietic disorders. However, immunodeficiencies following BMT continue to cause significant morbidity and mortality. We have compared the T cell receptor (TCR) repertoire of BMT patients and healthy control individuals by staining peripheral blood mononuclear cells with fluorochrome-labeled TCR-specific antibodies. Several patients exhibited a biased pattern of TCR expression atypical of the healthy controls, yet no particular TCR bias characterized all patients. For example, we found that 2%-8% of T cells from healthy individuals expressed the V beta 19 TCR. One BMT patient exhibited V beta 19 expression on more than 60% of peripheral T cells, while additional patients expressed V beta 19 on less than 1% of T cells. The patients with the most extreme skewing of TCR types suffered from graft-versus-host disease. The causes of skewed TCR V beta expression patterns in BMT patients are not fully understood, yet some researchers have suggested that an oligoclonal expansion of CD8+ T cell populations may be largely responsible. To test this hypothesis, we examined the TCR V beta repertoire of CD4+ and CD8+ T cell populations. We found that biased V beta expression characterized both CD4+ and CD8+ T cell populations, sometimes within a single individual. Thus, therapies directed toward CD8+ T cells alone may not fully correct repertoire abnormalities following BMT.
- Subjects :
- Adult
CD4-Positive T-Lymphocytes
Cancer Research
Adolescent
CD3 Complex
T cell
Receptors, Antigen, T-Cell, alpha-beta
Immunology
CD4-CD8 Ratio
chemical and pharmacologic phenomena
Biology
CD8-Positive T-Lymphocytes
medicine
Immunology and Allergy
Cytotoxic T cell
Humans
Transplantation, Homologous
Child
Bone Marrow Transplantation
T-cell receptor
Infant
hemic and immune systems
Receptors, Antigen, T-Cell, gamma-delta
T lymphocyte
medicine.disease
Leukemia
medicine.anatomical_structure
Graft-versus-host disease
Oncology
Child, Preschool
Bone marrow
CD8
Subjects
Details
- ISSN :
- 03407004
- Volume :
- 41
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cancer immunology, immunotherapy : CII
- Accession number :
- edsair.doi.dedup.....4d8b8675982b985691742ed096b2d74e