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Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine
- Source :
- Molecules, Molecules, Vol 23, Iss 8, p 1971 (2018), Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, Volume 23, Issue 8
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- Screens of antioxidant activity (AA) of various natural products have been a focus of the research community worldwide. This work aimed to differentiate selected samples of Merlot wines originated from Montenegro, with regard to phenolic profile and antioxidant capacity studied by survival rate, total sulfhydryl groups and activities of glutathione peroxidase (GPx), glutathione reductase and catalase in H2O2&ndash<br />stressed Saccharomyces cerevisiae cells. In this study, DPPH assay was also performed. Higher total phenolic content leads to an enhanced AA under both conditions. The same trend was observed for catechin and gallic acid, the most abundant phenolics in the examined wine samples. Finally, the findings of an Artificial Neural Network (ANN) model were in a good agreement (r2 = 0.978) with the experimental data. All tested samples exhibited a protective effect in H2O2&ndash<br />stressed yeast cells. Pre-treatment with examined wines increased survival in H2O2&ndash<br />stressed cells and shifted antioxidative defense towards GPx&ndash<br />mediated defense. Finally, sensitivity analysis of obtained ANN model highlights the complexity of the impact that variations in the concentrations of specific phenolic components have on the antioxidant defense system.
- Subjects :
- Antioxidant
DPPH
medicine.medical_treatment
Glutathione reductase
antioxidant activity
Pharmaceutical Science
Wine
Saccharomyces cerevisiae
01 natural sciences
Antioxidants
Article
Analytical Chemistry
Anthocyanins
lcsh:QD241-441
chemistry.chemical_compound
Picrates
lcsh:Organic chemistry
Drug Discovery
medicine
Sulfhydryl Compounds
Gallic acid
Food science
Physical and Theoretical Chemistry
chemistry.chemical_classification
Principal Component Analysis
010405 organic chemistry
Chemistry
Glutathione peroxidase
Biphenyl Compounds
010401 analytical chemistry
Organic Chemistry
Polyphenols
polyphenolics
Catechin
red wine
0104 chemical sciences
3. Good health
Chemistry (miscellaneous)
Polyphenol
Molecular Medicine
Neural Networks, Computer
artificial neural network
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....4d88e89d423ad352b33be002479813d5