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Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes
- Publication Year :
- 2012
-
Abstract
- Background Characterization of the immunoglobulin gene repertoire has improved our understanding of the immunopathogenesis of lymphoid tumors. Early B-lymphocyte precursors of multiple myeloma are known to exist and might be susceptible to antigenic drive. Design and Methods To verify this hypothesis, we collected a database of 345 fully readable multiple myeloma immunoglobulin sequences. We characterized the immunoglobulin repertoire, analyzed the somatic hypermutation load, and investigated for stereotyped receptor clusters. Results Compared to the normal immunoglobulin repertoire, multiple myeloma displayed only modest differences involving only a few genes, showing that the myeloma immunoglobulin repertoire is the least skewed among mature B-cell tumors. Median somatic hypermutation load was 7.8%; median length of complementarity determining-region 3 was 15.5 amino acids. Clustering analysis showed the absence of myeloma specific clusters and no similarity with published chronic lymphocytic leukemia or lymphoma subsets. Conclusions Analysis of multiple myeloma immunoglobulin repertoire does not support a pathogenetic role for antigen selection in this tumor.
- Subjects :
- Immunoglobulin gene
Myeloma protein
Chronic lymphocytic leukemia
Genes, Immunoglobulin Heavy Chain
Somatic hypermutation
Biology
hemic and lymphatic diseases
medicine
Data Mining
Humans
Multiple myeloma
B-Lymphocytes
Multiple Mieloma
Immunoglobulin genes
sequencing
Repertoire
Hematology
Sequence Analysis, DNA
medicine.disease
Complementarity Determining Regions
Lymphoma
Myeloma Proteins
Multigene Family
Immunology
biology.protein
Somatic Hypermutation, Immunoglobulin
Antibody
Original Articles and Brief Reports
Databases, Nucleic Acid
Multiple Myeloma
Settore MED/15 - Malattie del Sangue
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....4d73ff6c60a348972091b3b4d3800ef8