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Nascent Polypeptide-Associated Complex Involved in the Development and Pathogenesis of Fusarium graminearum on Wheat
- Source :
- Engineering, Vol 6, Iss 5, Pp 546-552 (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Reliable knowledge on pathogenic agents contributes to effective plant protection. For most plant pathogens, maintaining protein homeostasis (proteostasis) is essential for unfolding the cellular functions to survive and thrive. However, the fungal proteins involved in proteostasis remain poorly characterized in the process of pathogenesis. In this study, we characterized the function of the nascent polypeptide-associated complex (NAC) in Fusarium graminearum (F. graminearum) (FgNAC), one of the top 10 fungal pathogens with predominant scientific/economic importance. We found that FgNACα, a subunit of FgNAC, manifests high structural and functional similarity to its homologous counterparts in yeast and other species. The mutants of F. graminearum lacking NACα are viable but suffer significant defects in vegetative growth, conidial production, and pathogenesis. In addition, we show here that FgNACα can interact with another subunit of NAC (FgNACβ) in a yeast-two-hybrid assay. The subcellular localization results show that FgNACα and FgNACβ are predominantly localized in the cytoplasm. Future studies should focus on deciphering the mechanism by which NAC orchestrates protein biogenesis and consequentially modulates development and pathogenesis.
- Subjects :
- Environmental Engineering
General Computer Science
Materials Science (miscellaneous)
General Chemical Engineering
Protein subunit
Mutant
Energy Engineering and Power Technology
02 engineering and technology
Biology
010402 general chemistry
01 natural sciences
Gene knockout
Pathogenesis
Pathogenicity
Fungal protein
Subcellular localization
Nascent polypeptide-associated complex
General Engineering
food and beverages
021001 nanoscience & nanotechnology
0104 chemical sciences
Cell biology
Proteostasis
Fusarium head blight
lcsh:TA1-2040
0210 nano-technology
lcsh:Engineering (General). Civil engineering (General)
Function (biology)
Biogenesis
Subjects
Details
- Language :
- English
- ISSN :
- 20958099
- Volume :
- 6
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Engineering
- Accession number :
- edsair.doi.dedup.....4d71de2e04b856ba6510a4a524c04b67