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Molecular anatomy of ascending aorta in atherosclerosis by MS Imaging: Specific lipid and protein patterns reflect pathology
- Source :
- Journal of Proteomics, 126, 245-251
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- The molecular anatomy of healthy and atherosclerotic tissue is pursued here to identify ongoing molecular changes in atherosclerosis development. Subclinical atherosclerosis cannot be predicted and novel therapeutic targets are needed. Mass spectrometry imaging (MSI) is a novel unexplored ex vivo imaging approach in CVD able to provide in-tissue molecular maps. A rabbit model of early atherosclerosis was developed and high-spatial-resolution MALDI-MSI was applied to comparatively analyze histologically-based arterial regions of interest from control and early atherosclerotic aortas. Specific protocols were applied to identify lipids and proteins significantly altered in response to atherosclerosis. Observed protein alterations were confirmed by immunohistochemistry in rabbit tissue, and additionally in human aortas. Molecular features specifically defining different arterial regions were identified. Localized in the intima, increased expression of SFA and lysolipids and intimal spatial organization showing accumulation of PI, PG and SM point to endothelial dysfunction and triggered inflammatory response. TG, PA, SM and PE-Cer were identified specifically located in calcified regions. Thymosin β4 (TMSB4X) protein was upregulated in intima versus media layer and also in response to atherosclerosis. This overexpression and localization was confirmed in human aortas. In conclusion, molecular histology by MS Imaging identifies spatial organization of arterial tissue in response to atherosclerosis.
- Subjects :
- Proteomics
Pathology
medicine.medical_specialty
Biophysics
Molecular imaging
Biochemistry
Mass Spectrometry
Mass spectrometry imaging
Downregulation and upregulation
medicine
Animals
Humans
MALDI-MSI
Endothelial dysfunction
Vascular Calcification
Aorta
business.industry
Histology
Anatomy
Thymosin beta A
Atherosclerosis
medicine.disease
Lipids
Thymosin
Disease Models, Animal
Immunohistochemistry
Rabbits
Tunica Intima
business
Ex vivo
Subjects
Details
- ISSN :
- 18743919
- Volume :
- 126
- Database :
- OpenAIRE
- Journal :
- Journal of Proteomics
- Accession number :
- edsair.doi.dedup.....4d4f9b84df2de2993acfad98b9bcc16b