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Measuring Bovine γδ T Cell Function at the Site of Mycobacterium bovis Infection

Authors :
Mitchell V. Palmer
W. Ray Waters
Rachel A. Rusk
Jodi L. McGill
Publication Year :
2017

Abstract

Bovine γδ T cells are amongst the first cells to accumulate at the site of Mycobacterium bovis infection; however, their role in the developing lesion remains unclear. We utilized transcriptomics analysis, in situ hybridization, and a macrophage/γδ T cell co-culture system to elucidate the role of γδ T cells in local immunity to M. bovis infection. Transcriptomics analysis revealed that γδ T cells upregulated expression of several novel, immune-associated genes in response to stimulation with M. bovis antigen. BCG-infected macrophage/γδ T cell co-cultures confirmed the results of our RNAseq analysis, and revealed that γδ T cells from M. bovis-infected animals had a significant impact on bacterial viability. Analysis of γδ T cells within late-stage M. bovis granulomas revealed significant expression of IFN-γ and CCL2, but not IL-10, IL-22, or IL-17. Our results suggest γδ T cells influence local immunity to M. bovis through cytokine secretion and direct effects on bacterial burden.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....4d4d49dbe94eb73817d325a44657fae6