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Effect of particle size on the biodistribution of lipid nanocapsules: Comparison between nuclear and fluorescence imaging and counting
- Source :
- International Journal of Pharmaceutics, International Journal of Pharmaceutics, Elsevier, 2013, 453 (2), pp.594-600. 〈10.1016/j.ijpharm.2013.05.057〉, International Journal of Pharmaceutics, Elsevier, 2013, 453 (2), pp.594-600. ⟨10.1016/j.ijpharm.2013.05.057⟩, International Journal of Pharmaceutics, 2013, 453 (2), pp.594-600. ⟨10.1016/j.ijpharm.2013.05.057⟩
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- International audience; In vivo biodistribution of nanoparticles depends on several physicochemical parameters such as size. After intravenous injection of 25, 50 and 100nm lipid nanocapsules (LNC) in nude mice bearing HEK293(β3) tumour xenografts, biodistribution was evaluated by γ-scintigraphy and by γ-counting. The small LNC 25nm disappeared faster than the larger LNC 50 and 100nm from the blood circulation due to faster elimination and wider tissue distribution. At 24h, biodistribution profiles of all these LNC were similar. Low LNC quantities were found in this weak EPR (enhanced permeability and retention) tumour regardless the particle size. Co-injected 50nm fluorescent DiD-LNC and (99m)Tc-LNC allowed direct comparison of biodistribution as evaluated by the two methods. Optical imaging underestimated LNC quantity especially in dark-colored organs that were observed to capture extensive quantities of the particles by γ-counting (i.e. liver, spleen, and kidney).
- Subjects :
- Biodistribution
Fluorescence-lifetime imaging microscopy
Lipid nanocapsules
γ-scintigraphy
Analytical chemistry
Pharmaceutical Science
Nanoparticle
Mice, Nude
02 engineering and technology
[SDV.IB.MN]Life Sciences [q-bio]/Bioengineering/Nuclear medicine
[SDV.IB.MN] Life Sciences [q-bio]/Bioengineering/Nuclear medicine
[ SDV.IB.MN ] Life Sciences [q-bio]/Bioengineering/Nuclear medicine
03 medical and health sciences
Mice
optical imaging
Optical imaging
Nanocapsules
Animals
Humans
Tissue Distribution
Particle Size
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
Radionuclide Imaging
biodistribution
030304 developmental biology
0303 health sciences
lipid nanocapsules
Chemistry
Technetium
021001 nanoscience & nanotechnology
[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterials
Fluorescence
Lipids
[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials
HEK293 Cells
Permeability (electromagnetism)
Biophysics
Scintillation Counting
Female
Particle size
fluorescence
0210 nano-technology
Subjects
Details
- Language :
- English
- ISSN :
- 03785173
- Database :
- OpenAIRE
- Journal :
- International Journal of Pharmaceutics, International Journal of Pharmaceutics, Elsevier, 2013, 453 (2), pp.594-600. 〈10.1016/j.ijpharm.2013.05.057〉, International Journal of Pharmaceutics, Elsevier, 2013, 453 (2), pp.594-600. ⟨10.1016/j.ijpharm.2013.05.057⟩, International Journal of Pharmaceutics, 2013, 453 (2), pp.594-600. ⟨10.1016/j.ijpharm.2013.05.057⟩
- Accession number :
- edsair.doi.dedup.....4d4c05ad338e6a1257a2bba3ee51617c