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Involvement of signal peptidase I in Streptococcus sanguinis biofilm formation
- Source :
- Microbiology
- Publication Year :
- 2017
-
Abstract
- Biofilm accounts for 65-80 % of microbial infections in humans. Considerable evidence links biofilm formation by oral microbiota to oral disease and consequently systemic infections. Streptococcus sanguinis, a Gram-positive bacterium, is one of the most abundant species of the oral microbiota and it contributes to biofilm development in the oral cavity. Due to its altered biofilm formation, we investigated a biofilm mutant, ΔSSA_0351, that is deficient in type I signal peptidase (SPase) in this study. Although the growth curve of the ΔSSA_0351 mutant showed no significant difference from that of the wild-type strain SK36, biofilm assays using both microtitre plate assay and confocal laser scanning microscopy (CLSM) confirmed a sharp reduction in biofilm formation in the mutant compared to the wild-type strain and the paralogous mutant ΔSSA_0849. Scanning electron microscopy (SEM) revealed remarkable differences in the cell surface morphologies and chain length of the ΔSSA_0351 mutant compared with those of the wild-type strain. Transcriptomic and proteomic assays using RNA sequencing and mass spectrometry, respectively, were conducted on the ΔSSA_0351 mutant to evaluate the functional impact of SPase on biofilm formation. Subsequently, bioinformatics analysis revealed a number of proteins that were differentially regulated in the ΔSSA_0351 mutant, narrowing down the list of SPase substrates involved in biofilm formation to lactate dehydrogenase (SSA_1221) and a short-chain dehydrogenase (SSA_0291). With further experimentation, this list defined the link between SSA_0351-encoded SPase, cell wall biosynthesis and biofilm formation.
- Subjects :
- 0301 basic medicine
Proteomics
Physiology and Metabolism
030106 microbiology
Mutant
Dehydrogenase
Microbiology
biofilm
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
Lactate dehydrogenase
Databases, Genetic
Data Mining
mass spectrometry
Signal peptidase
Strain (chemistry)
biology
Gene Expression Profiling
Serine Endopeptidases
Biofilm
Computational Biology
Membrane Proteins
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Molecular biology
Streptococcus sanguinis
chemistry
Biofilms
Mutation
signal peptidase
Cues
Streptococcus sanguis
RNA-seq
Bacteria
Research Article
Subjects
Details
- ISSN :
- 14652080
- Volume :
- 163
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Microbiology (Reading, England)
- Accession number :
- edsair.doi.dedup.....4d46622e736545a5998820f84814db2e