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The involvement of Fas/FasL interaction in porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus co-inoculation-associated lymphocyte apoptosis in vitro
- Source :
- Veterinary microbiology. 122(1-2)
- Publication Year :
- 2006
-
Abstract
- Lymphoid depletion of various lymphoid organs is one of the major lesions in pigs suffering from postweaning multisystemic wasting syndrome (PMWS). The co-existence of porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) in PMWS-affected pigs along with the more severe and wider range of lymphocyte depletion of lymphoid organs in PCV2 and PRRSV dually-inoculated pigs imply that PCV2 and PRRSV may interact in the pathogenesis of PMWS. The mechanism for the development of lymphoid depletion in PMWS-affected pigs remains controversial. The objective of the present study was to evaluate and compare the effects of inoculation of both viruses, singularly or in combination, on swine splenic macrophages (SMs) and co-cultured splenic (SLs) and peripheral blood (PBLs) lymphocytes in vitro. A significant reduction in the survival rate and increase in the apoptotic rate of the co-cultured SLs and PBLs and concurrent increase in the expression levels of Fas ligand (FasL) in SMs and Fas in co-cultured SLs and PBLs were demonstrated in PRRSV alone- and PCV2 and PRRSV dually-inoculated groups with the latter more prominent. The increased FasL was proven capable of inducing Fas/FasL-mediated apoptosis in co-cultured FasL-sensitive Jurkat T cells. The de novo expression and production of FasL in PCV2 and PRRSV dually-inoculated SMs and concurrently increased surface expression of Fas in co-cultured lymphocytes may contribute, at least partially, to lymphoid depletion in PMWS-affected pigs with PCV2 and PRRSV dual infection.
- Subjects :
- Circovirus
Male
Fas Ligand Protein
Swine
animal diseases
Lymphocyte
Apoptosis
Microbiology
Fas ligand
Arterivirus
Jurkat Cells
medicine
Animals
Humans
Porcine respiratory and reproductive syndrome virus
Lymphocytes
RNA, Messenger
fas Receptor
General Veterinary
biology
Macrophages
virus diseases
General Medicine
biology.organism_classification
Porcine reproductive and respiratory syndrome virus
Virology
Porcine circovirus
medicine.anatomical_structure
Gene Expression Regulation
Immunology
Female
Circoviridae
Spleen
Subjects
Details
- ISSN :
- 03781135
- Volume :
- 122
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Veterinary microbiology
- Accession number :
- edsair.doi.dedup.....4d2cdea96e6bb3feaef930822a98a342