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Ultra-small particles of iron oxide as peroxidase for immunohistochemical detection
- Source :
- Nanotechnology. 22(22)
- Publication Year :
- 2011
-
Abstract
- Dimercaptosuccinic acid (DMSA) modified ultra-small particles of iron oxide (USPIO) were synthesized through a two-step process. The first step: oleic acid (OA) capped Fe(3)O(4) (OA-USPIO) were synthesized by a novel oxidation coprecipitation method in H(2)O/DMSO mixing system, where DMSO acts as an oxidant simultaneously. The second step: OA was replaced by DMSA to obtain water-soluble nanoparticles. The as-synthesized nanoparticles were characterized by TEM, FTIR, TGA, VSM, DLS, EDS and UV-vis. Hydrodynamic sizes and Peroxidase-like catalytic activity of the nanoparticles were investigated. The hydrodynamic sizes of the nanoparticles (around 24.4 nm) were well suited to developing stable nanoprobes for bio-detection. The kinetic studies were performed to quantitatively evaluate the catalytic ability of the peroxidase-like nanoparticles. The calculated kinetic parameters indicated that the DMSA-USPIO possesses high catalytic activity. Based on the high activity, immunohistochemical experiments were established: using low-cost nanoparticles as the enzyme instead of expensive HRP, Nimotuzumab was conjugated onto the surface of the nanoparticles to construct a kind of ultra-small nanoprobe which was employed to detect epidermal growth factor receptor (EGFR) over-expressed on the membrane of esophageal cancer cell. The proper sizes of the probes and the result of membranous immunohistochemical staining suggest that the probes can be served as a useful diagnostic reagent for bio-detection.
- Subjects :
- Esophageal Neoplasms
Coprecipitation
Inorganic chemistry
Iron oxide
Nanoprobe
Nanoparticle
Bioengineering
Conjugated system
Ferric Compounds
Catalysis
chemistry.chemical_compound
Cell Line, Tumor
Humans
General Materials Science
Electrical and Electronic Engineering
Particle Size
Peroxidase
Mechanical Engineering
General Chemistry
Immunohistochemistry
ErbB Receptors
Membrane
chemistry
Mechanics of Materials
Nanoparticles
Particle size
Succimer
Subjects
Details
- ISSN :
- 13616528
- Volume :
- 22
- Issue :
- 22
- Database :
- OpenAIRE
- Journal :
- Nanotechnology
- Accession number :
- edsair.doi.dedup.....4d0a49e342c7890a9b6c27466cb56725