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A metabolomics approach to studying the effects of Jinxin oral liquid on RSV-infected mice using UPLC/LTQ-Orbitrap mass spectrometry
- Source :
- Journal of Ethnopharmacology. 174:25-36
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Ethnopharmacological relevance Jinxin oral liquid (JOL) is a traditional Chinese medicine (TCM) formula modified from ma-xing-shi-gan-tang, an ancient formula widely used in the treatment of respiratory diseases such as bronchitis, pneumonia, and asthma. In our previous studies, JOL was shown to safely and effectively treat viral pneumonia, especially that involving respiratory syncytial virus (RSV). Aim of the study To investigate the mechanism of the effect of JOL in RSV infected mice, using a metabolomics approach based on ultra-performance liquid chromatography coupled with linear ion trap quadrupole-Orbitrap mass spectrometry (UPLC/LTQ-Orbitrap–MS). Materials and methods BALB/c mice were divided into four groups, the control group (saline inoculation/no treatment), RSV group (RSV inoculation/saline treatment), RSV+JOL group (RSV inoculation/JOL treatment), and RSV+Riba group (RSV inoculation/ribavirin treatment). Plasma and lung tissue samples were collected 7 days after the inoculation/treatment protocols, and UPLC/LTQ-Orbitrap-MS method based on metabolomics was developed. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were utilized to identify biomarkers potentially associated with the anti-RSV activity of JOL. Results JOL was associated with reduced inflammatory responses in RSV-infected lung tissue. The combination of PCA and OPLS-DA revealed deviations in 11 biomarkers in plasma, and 16 biomarkers in lung tissue induced by RSV that were corrected with JOL treatment. These biomarkers were primarily components of metabolic pathways involving glycerophosphocholines, sphingolipids, and glycerolipids. JOL was able to restore the abnormal levels of these biomarkers detected in the plasma and lung tissue of RSV-infected mice to approximately normal levels. Conclusions This study suggested that JOL can treat RSV pneumonia effectively, partially by ameliorating the associated disturbances to lipid metabolism. The results provided insight into the anti-RSV mechanism of JOL, and also demonstrated that metabolomics is a valuable tool for investigating the efficacy of TCM treatment for RSV pneumonia, and the associated biomarkers involved.
- Subjects :
- viruses
Administration, Oral
Respiratory Syncytial Virus Infections
Mass Spectrometry
Virus
Cell Line
Mice
chemistry.chemical_compound
Metabolomics
Tandem Mass Spectrometry
Liquid chromatography–mass spectrometry
Drug Discovery
medicine
Animals
Humans
Respiratory system
Chromatography, High Pressure Liquid
Pharmacology
Mice, Inbred BALB C
Ribavirin
respiratory system
medicine.disease
Respiratory Syncytial Viruses
Pharmaceutical Solutions
Pneumonia
chemistry
Viral pneumonia
Immunology
Bronchitis
Female
Chromatography, Liquid
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 03788741
- Volume :
- 174
- Database :
- OpenAIRE
- Journal :
- Journal of Ethnopharmacology
- Accession number :
- edsair.doi.dedup.....4d06e9dab6cd9f0889390a76189cd4ab