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Cell-type-specific chromatin occupancy by the pioneer factor Zelda drives key developmental transitions in Drosophila
- Source :
- Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021), Nature Communications
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- During Drosophila embryogenesis, the essential pioneer factor Zelda defines hundreds of cis-regulatory regions and in doing so reprograms the zygotic transcriptome. While Zelda is essential later in development, it is unclear how the ability of Zelda to define cis-regulatory regions is shaped by cell-type-specific chromatin architecture. Asymmetric division of neural stem cells (neuroblasts) in the fly brain provide an excellent paradigm for investigating the cell-type-specific functions of this pioneer factor. We show that Zelda synergistically functions with Notch to maintain neuroblasts in an undifferentiated state. Zelda misexpression reprograms progenitor cells to neuroblasts, but this capacity is limited by transcriptional repressors critical for progenitor commitment. Zelda genomic occupancy in neuroblasts is reorganized as compared to the embryo, and this reorganization is correlated with differences in chromatin accessibility and cofactor availability. We propose that Zelda regulates essential transitions in the neuroblasts and embryo through a shared gene-regulatory network driven by cell-type-specific enhancers.<br />The pioneer transcription factor Zelda is essential for the reprogramming of germ cells to totipotent cells of the early Drosophila embryo. Here the authors examine the function of Zelda later in development in the larval brain to show that Zelda promotes the undifferentiated stem-cell fate. They further identify factors that limit the reprogramming capacity of this pioneer factor.
- Subjects :
- animal structures
Science
General Physics and Astronomy
Embryonic Development
Biology
General Biochemistry, Genetics and Molecular Biology
Article
Neuroblast
Animals
Drosophila Proteins
Progenitor cell
Enhancer
Progenitor
Multidisciplinary
Receptors, Notch
Stem Cells
Pioneer factor
fungi
Drosophila embryogenesis
Gene Expression Regulation, Developmental
Nuclear Proteins
Cell Differentiation
General Chemistry
Neural stem cell
Chromatin
humanities
Cell biology
Drosophila melanogaster
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....4cff74791fd570b7710cf0fd5ea3b13e