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γ-H2AX as a Marker for Dose Deposition in the Brain of Wistar Rats after Synchrotron Microbeam Radiation
- Source :
- PLoS ONE, PLoS ONE, Vol 10, Iss 3, p e0119924 (2015), 'PloS One ', vol: 10, pages: e0119924-1-e0119924-17 (2015), PLoS ONE, Public Library of Science, 2015, 10 (3), 17 p. ⟨10.1371/journal.pone.0119924⟩, Fernandez Palomo, Cristian; Mothersill, Carmel; Bräuer-Krisch, Elke; Laissue, Jean; Seymour, Colin; Schültke, Elisabeth (2015). γ-H2AX as a Marker for Dose Deposition in the Brain of Wistar Rats after Synchrotron Microbeam Radiation. PLoS ONE, 10(3), e0119924. Public Library of Science 10.1371/journal.pone.0119924
- Publication Year :
- 2015
- Publisher :
- Public Library of Science, 2015.
-
Abstract
- International audience; Objective Synchrotron radiation has shown high therapeutic potential in small animal models of malignant brain tumours. However, more studies are needed to understand the radiobiological effects caused by the delivery of high doses of spatially fractionated x-rays in tissue. The purpose of this study was to explore the use of the gamma-H2AX antibody as a marker for dose deposition in the brain of rats after synchrotron microbeam radiation therapy (MRT). Methods Normal and tumour-bearing Wistar rats were exposed to 35, 70 or 350 Gy of MRT to their right cerebral hemisphere. The brains were extracted either at 4 or 8 hours after irradiation and immediately placed in formalin. Sections of paraffin-embedded tissue were incubated with anti gamma-H2AX primary antibody. Results While the presence of the C6 glioma does not seem to modulate the formation of gamma-H2AX in normal tissue, the irradiation dose and the recovery versus time are the most important factors affecting the development of gamma-H2AX foci. Our results also suggest that doses of 350 Gy can trigger the release of bystander signals that significantly amplify the DNA damage caused by radiation and that the gamma-H2AX biomarker does not only represent DNA damage produced by radiation, but also damage caused by bystander effects. Conclusion In conclusion, we suggest that the gamma-H2AX foci should be used as biomarker for targeted and non-targeted DNA damage after synchrotron radiation rather than a tool to measure the actual physical doses
- Subjects :
- Male
Pathology
medicine.medical_specialty
DNA damage
medicine.medical_treatment
[SDV]Life Sciences [q-bio]
Science
Synchrotron radiation
Biology
Histones
Immunoenzyme Techniques
Glioma
Bystander effect
medicine
Image Processing, Computer-Assisted
Tumor Cells, Cultured
Animals
Irradiation
Rats, Wistar
Multidisciplinary
Brain Neoplasms
X-Rays
Radiotherapy Dosage
Microbeam
Bystander Effect
medicine.disease
Phosphoproteins
3. Good health
Rats
Radiation therapy
Biomarker (medicine)
Medicine
Biomarkers
Synchrotrons
Research Article
DNA Damage
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....4cfd6c0b0517a768ce346ea67b86cfb7