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The Role of B Cells in in Vitro Induction of IFN-γ-Producing CD4+ T Cells Specific to Listeria monocytogens: Positive and IL-10-Mediated Negative Regulation
- Source :
- Cellular Immunology. 157:403-414
- Publication Year :
- 1994
- Publisher :
- Elsevier BV, 1994.
-
Abstract
- We have reported that Listeria monocytogenes-specific IFN-gamma-producing CD4+ T cells are induced by in vitro 5-day culture of naive spleen cells with viable L. monocytogenes (VLM), but not induced by culture with heat-killed L. monocytogenes (HKLM). In the present study, the role of B cells in the regulation of induction of IFN-gamma-producing CD4+ T cells in the in vitro system was investigated. We found that L. monocytogenes-specific IFN-gamma-producing CD4+ T cells were not generated when B cells were depleted from spleen cells before culture with VLM although Ag-specific proliferative response was retained. IFN-gamma production by CD4+ T cells was restored by addition of B cells cultured with VLM to the culture of B cell-depleted spleen cells and VLM. In contrast, B cells cultured with HKLM could not restore the induction of IFN-gamma production when added in culture of B cell-depleted spleen cells and VLM. Analysis of cytokine gene expression by reverse transcription-polymerase chain reaction method revealed that expression of interleukin-10 (IL-10) was higher but TNF-alpha was lower in B cells cultured with HKLM when compared with that in B cells cultured with VLM. Furthermore, addition of neutralizing anti-IL-10 mAb into culture of naive spleen cells with HKLM resulted in appearance of IFN-gamma-producing cells. These results suggest that B cells have positive and negative roles in the induction of IFN-gamma-producing CD4+ T cells. The inhibition of induction of IFN-gamma-producing CD4+ T cells may depend on B cell-derived IL-10.
- Subjects :
- CD4-Positive T-Lymphocytes
Molecular Sequence Data
Immunology
Gene Expression
Biology
Lymphocyte Depletion
Interferon-gamma
Mice
Interleukin 21
Animals
Cytotoxic T cell
RNA, Messenger
IL-2 receptor
Antigen-presenting cell
DNA Primers
Interleukin 3
Antigens, Bacterial
B-Lymphocytes
Mice, Inbred C3H
CD40
Base Sequence
Listeria monocytogenes
Molecular biology
Interleukin-10
Cell biology
Interleukin 10
biology.protein
Interleukin 12
Cytokines
Female
Spleen
Subjects
Details
- ISSN :
- 00088749
- Volume :
- 157
- Database :
- OpenAIRE
- Journal :
- Cellular Immunology
- Accession number :
- edsair.doi.dedup.....4cf9cd9a717c98e6bd41055198a44948
- Full Text :
- https://doi.org/10.1006/cimm.1994.1237