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Thrombopoietin could protect cerebral tissue against ischemia-reperfusion injury by suppressing NF-κB and MMP-9 expression in rats
- Source :
- International Journal of Medical Sciences
- Publication Year :
- 2018
-
Abstract
- Objective: To determine the neuroprotective effects and underpinning mechanisms of thrombopoietin (TPO), Matrix Metalloproteinase-9(MMP-9) and Nuclear Factor-κB (NF-κB) after focal cerebral ischemia-reperfusion in rats. Methods: Male rats underwent 2 hours of right middle cerebral artery occlusion (MCAO) followed by 22 hours of reperfusion. PBS or TPO (0.1ug/kg) was administered from caudal vein before reperfusion. Neurologic deficits, brain edema, Evans blue (EB) extravasation, NF-κB and MMP-9 expression were subsequently examined. Results: Ischemia-reperfusion injury produced a large area of edema. TPO significantly reduced edema and alleviated neurologic deficits after ischemia-reperfusion. Ischemia-induced increases of NF-κB, MMP-9 and Evans blue extravasation were reduced by TPO intervention. Conclusion: TPO improved neurological function and ameliorated brain edema after stroke, partly by reducing the ischemia-induced increase of NF-κB and MMP-9.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Ischemia
Brain Edema
Neuroprotection
Matrix Metalloproteinase-9 (MMP-9)
Thrombopoietin (TPO)
Brain Ischemia
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Nuclear factor-κB (NF-κB)
Edema
Internal medicine
Ischemia-Reperfusion (IR)
medicine
Animals
Stroke
Thrombopoietin
Evans Blue
business.industry
NF-kappa B
General Medicine
medicine.disease
Extravasation
Rats
030104 developmental biology
Endocrinology
Neuroprotective Agents
chemistry
Matrix Metalloproteinase 9
Blood-Brain Barrier
Reperfusion Injury
medicine.symptom
business
Reperfusion injury
030217 neurology & neurosurgery
Research Paper
Subjects
Details
- ISSN :
- 14491907
- Volume :
- 15
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- International journal of medical sciences
- Accession number :
- edsair.doi.dedup.....4cf963c89e34fbfd1d9e9ed5300ca015