Back to Search
Start Over
DNA TYPING OF HLA-DQ ALLELES BY GENE AMPLIFICATION OF DQA AND DQB VARIABLE EXONS: ANALYSIS OF DQA/DQB HAPLOTYPES
- Source :
- Scopus-Elsevier
- Publication Year :
- 1992
- Publisher :
- Wiley, 1992.
-
Abstract
- In the present report, we describe a DNA typing method that allows detection of all the polymorphic variants of DQA1 and DQB1 second exons. By the oligotyping procedure provided in this paper, we are able to identify 8 DQA1 and 13 DQB1 alleles and to type random individuals in any heterozygous combination. We provide the hybridization and washing temperatures for using either 32P labelled or non-radioactive probes. The discrimination power of this procedure, compared to serological and cellular techniques, is remarkable. Therefore, this typing method finds perfect application in transplantation immunology and it will be very helpful to optimize the matching of unrelated donors before BMT. It is apparent from our results that despite the linkage disequilibrium present between DQ and DR loci, a DR specificity may frequently be associated to different DQ haplotypes. This is the case for DR4, DR7, DR8, DR9, and DR13 specificities.
- Subjects :
- musculoskeletal diseases
endocrine system diseases
Molecular Sequence Data
Immunology
Biology
HLA-DQ alpha-Chains
Linkage Disequilibrium
law.invention
Gene Frequency
law
HLA-DQ Antigens
HLA-DQ
Gene duplication
Genetics
HLA-DQ beta-Chains
Humans
Typing
Allele
Gene
Alleles
Polymerase chain reaction
Base Sequence
Histocompatibility Testing
Haplotype
Gene Amplification
nutritional and metabolic diseases
DNA
Exons
DNA Probes, HLA
Transplantation
Haplotypes
Italy
Subjects
Details
- ISSN :
- 13652370 and 09607420
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunogenetics
- Accession number :
- edsair.doi.dedup.....4cf044493bc5850681202bb5c93072f9