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Fast and efficient analyses of the post-mortem human blood and bone marrow using DI-SPME/LC-TOFMS method for forensic medicine purposes
- Source :
- Talanta. 209
- Publication Year :
- 2019
-
Abstract
- For the first time the method DI-SPME/LC-TOFMS was used and developed in order to determine the large antidepressant drugs in real forensic cases. The aim of the study was to optimize the new DI-SPME/LC-TOFMS method for the quantification of the large group of psychotropic drugs such as benzodiazepines, selective serotonin reuptake inhibitors, selective serotonin and noradrenaline reuptake inhibitors, tricyclic antidepressants and sleeping pills "Z". The volume of the sample, adsorption time, post-adsorption purification and desorption time were precisely optimized. The validation parameters such as limit of detection and quantification, linearity, precision during and between days and the matrix effect were determined. All obtained values are within the acceptable range for toxicological analyses. The usefulness of the method was confirmed by analyzing the post-mortem samples. Drug concentrations were determined in real samples with high precision, which gives perspectives for the DI-SPME/LC-TOFMS routine application in toxicological and forensic analyses in the future.
- Subjects :
- Time Factors
02 engineering and technology
Noradrenaline reuptake
01 natural sciences
Mass Spectrometry
Analytical Chemistry
Desorption time
Bone Marrow
Limit of Detection
Humans
Solid Phase Microextraction
Detection limit
Psychotropic Drugs
Chromatography
Human blood
Chemistry
Lc tofms
010401 analytical chemistry
Serotonin reuptake
Forensic Medicine
021001 nanoscience & nanotechnology
Antidepressive Agents
0104 chemical sciences
Antidepressant
Autopsy
0210 nano-technology
Large group
Subjects
Details
- ISSN :
- 18733573
- Volume :
- 209
- Database :
- OpenAIRE
- Journal :
- Talanta
- Accession number :
- edsair.doi.dedup.....4cdfbfad0db3c35b8f008a6ef4ba654d