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The Neurovascular Protection Afforded by Delayed Local Hypothermia after Transient Middle Cerebral Artery Occlusion
- Source :
- Current Neurovascular Research. 10:134-143
- Publication Year :
- 2013
- Publisher :
- Bentham Science Publishers Ltd., 2013.
-
Abstract
- Therapeutic hypothermia is a robust therapeutic tool in experimental stroke models but its clinical applications are limited. Furthermore, optimal conditions for therapeutic hypothermia, such as, temperature and the initiation and duration of cooling must be individualized. Here, we evaluated the therapeutic effects of delayed local hypothermia, administered for 44 hr after 4 hr of reperfusion in a rat model of transient middle cerebral artery occlusion (tMCAo), using a cooling device that allowed controlled local hypothermia (31 °C) in brain. Histological data revealed that local hypothermia significantly reduced infarct volumes and glial hypertrophic activation. Brain water contents, IgG leakage, and Evans Blue extravasation were notably reduced by local hypothermia. Furthermore, local hypothermia had strong vasculoprotective effects, as determined by immunohistochemistry and Western blot analyses for endothelial barrier antigen (EBA), laminin, aquaporin-4, and tight junction proteins in brain. Our data indicate that delayed/prolonged local hypothermia confers neurovascular protection, reduces brain edema, and inhibits inflammatory glial activation, and suggest that hypothermic conservation of vascular structures and functions account for the therapeutic effects of local hypothermia observed in this model of experimental stroke.
- Subjects :
- Male
Blotting, Western
Infarction
Rats, Sprague-Dawley
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Developmental Neuroscience
Hypothermia, Induced
medicine
Animals
Evans Blue
Tight junction
business.industry
Therapeutic effect
Infarction, Middle Cerebral Artery
Hypothermia
Neurovascular bundle
medicine.disease
Immunohistochemistry
Extravasation
Rats
Disease Models, Animal
Neurology
chemistry
Reperfusion Injury
Anesthesia
medicine.symptom
business
Subjects
Details
- ISSN :
- 15672026
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Current Neurovascular Research
- Accession number :
- edsair.doi.dedup.....4cde2f3177400c3745301685c142b003