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Shogaol potentiates sevoflurane mediated neuroprotection against ischemia/reperfusion-induced brain injury via regulating apoptotic proteins and PI3K/Akt/mTOR/s6K signalling and HIF-1α/HO-1 expression
- Source :
- Saudi Journal of Biological Sciences, Vol 28, Iss 9, Pp 5002-5010 (2021), Saudi Journal of Biological Sciences
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- The current research was intended to evaluate the impact of 6-shogaol in rodent model of ischemic-reperfusion induced- brain injury and also assessed 6-shogaol enhanced sevoflurane's neuroprotective effects. Ischemic-Reperfusion (I/R) injury was induced by middle cerebral artery occlusion (MCAO) method in Sprague-Dawley rats. A separate group of animal was exposed to sevoflurane (2.5%) post-conditioning for 1 h immediately after reperfusion. The 6-shogaol (25 mg or 50 mg/kg body weight) was orally administered to treatment group rats for 14 days and then subjected to I/R. The 6-shogaol treatment along with/without sevoflurane post-conditioning reduced the number of apoptotic cell counts, brain edema and cerebral infarct volume. The western blotting analysis revealed a significant stimulation of the PI3K/Akt/mTOR signal pathway. RT-PCR and western blotting studies revealed improved expressions of HIF-1α and HO-1 at both gene level and protein levels. I/R induced neurological deficits were also alleviated on sevoflurane post-conditioning with/without 6-shogaol treatment. The present findings revealed that pre-treatment with 6-shogoal enhanced the neuroprotective properties of sevoflurane post-conditioning, illustrated the efficacy of the compound against I/R injury.
- Subjects :
- Hemeoxygenase 1
business.industry
QH301-705.5
Ischemia
P70-S6 Kinase 1
Stimulation
Pharmacology
medicine.disease
Neuroprotection
Sevoflurane
Apoptosis
Medicine
Hypoxia-inducible factor 1α
protein kinase B
Original Article
6-shogaol
Biology (General)
General Agricultural and Biological Sciences
business
Protein kinase B
PI3K/AKT/mTOR pathway
medicine.drug
Subjects
Details
- Language :
- English
- Volume :
- 28
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Saudi Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....4cc9f9aa5870e1bb68340e5965880fdd