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Dietary Fat and Sugar Differentially Affect β-Adrenergic Stimulation of Cardiac ERK and AKT Pathways in C57BL/6 Male Mice Subjected to High-Calorie Feeding
- Source :
- J Nutr
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- BACKGROUND: High dietary fat and sugar promote cardiac hypertrophy independently from an increase in blood pressure. The respective contribution that each macronutrient exerts on cardiac growth signaling pathways remains unclear. OBJECTIVE: The goal of this study was to investigate the mechanisms by which high amounts of dietary fat and sugar affect cardiac growth regulatory pathways. METHODS: Male C57BL/6 mice (9 wk old; n = 20/group) were fed a standard rodent diet (STD; kcal% protein-fat-carbohydrate, 29-17-54), a high-fat diet (HFD; 20-60-20), a high-fat and high-sugar Western diet (WD; 20-45-35), a high-sugar diet with mixed carbohydrates (HCD; 20-10-70), or a high-sucrose diet (HSD; 20-10-70). Body composition was assessed weekly by EchoMRI. Whole-body glucose utilization was assessed with an intraperitoneal glucose tolerance test. After 6 wk on diets, mice were treated with saline or 20 mg/kg isoproterenol (ISO), and the activity of cardiac growth regulatory pathways was analyzed by immunoblotting. Data were analyzed by ANOVA with data from the STD group included for references only. RESULTS: Compared with HCD and HSD, WD and HFD increased body fat mass 2.7- to 3.8-fold (P 1.5-fold (P 47%; P 34%; P 64%; P
- Subjects :
- Blood Glucose
Male
medicine.medical_specialty
Calorie
Sucrose
Dietary Sugars
MAP Kinase Signaling System
medicine.medical_treatment
Medicine (miscellaneous)
Mice
chemistry.chemical_compound
Overnutrition
Insulin, Regular, Human
Internal medicine
medicine
Animals
Protein kinase B
Glucose tolerance test
Nutrition and Dietetics
biology
medicine.diagnostic_test
business.industry
Insulin
Biochemical, Molecular, and Genetic Mechanisms
medicine.disease
Dietary Fats
Lipids
Mice, Inbred C57BL
Insulin receptor
Blood pressure
Endocrinology
Gene Expression Regulation
chemistry
biology.protein
Energy Intake
business
Proto-Oncogene Proteins c-akt
Subjects
Details
- ISSN :
- 00223166
- Volume :
- 150
- Database :
- OpenAIRE
- Journal :
- The Journal of Nutrition
- Accession number :
- edsair.doi.dedup.....4cc487a5e380a291ad40ef209e35a483