Bacterium isolated from coffee waste pulp biosorps lead: Investigation of EPS mediated mechanism

Highlights • Kleibsiella pneumoniae Kpn555 tolerates 900 mg/L lead. • SEM and TEM studies revealed surface deposition and bioaccumulation of lead. • Surface deposition mediated by EPS produced in response to lead stress, characterised as glycolipid with protein moieties. • Maximum biosorption ability of EPS – 475 mg/g. • Ability of lead bioaccumulation is plasmid mediated.
Kleibsiella pneumoniae Kpn555, isolated from coffee waste pulp showed high level of tolerance to lead with a minimum inhibitory concentration of 900 mg/L. On its growth in nutrient broth supplemented with lead, brown clumps were visualised at the bottom of the flask. On scanning and transmission electron microscopic studies the brown clumps were corroborated to be bacterial cells with lead biosorbed on the cell surface and accumulated inside the cytoplasm. Biochemical and FT-IR analysis of the extracellular polymeric substance produced on exposure to lead revealed its chemical nature as glycolipid with protein moieties. Purified EPS (100 mg/L) could remove 50% of lead from aqueous solution (200 mg/L). Isolation of plasmid from Klebsiella pneumoniae Kpn555 revealed the presence of a plasmid of size 30–40 kb. This capability of the bacteria was proven to be plasmid mediated as the Escherichia coli DH5α cells transformed with the plasmid of Klebsiella pneumoniae Kpn555 also could tolerate 900 mg/L of lead and form brown clumps. This study shows that these bacteria, aided by EPS could serve as an effective agent for the removal of lead from contaminated water environmental samples.
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