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A Sensitive Two-Analyte Immunochromatographic Strip for Simultaneously Detecting Aflatoxin M1 and Chloramphenicol in Milk
- Source :
- Toxins, Vol 12, Iss 637, p 637 (2020), Toxins, Volume 12, Issue 10
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- A two-analyte immunochromatographic strip (immunostrip) was developed for the simultaneous detection of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk. Protein conjugates (AFM1-ovalbumin (OVA) and CAP-OVA) and goat anti-rabbit IgG were respectively drawn on nitrocellulose membrane as two test lines (T1 and T2) and a control line (C). The immunostrip was dipped into a well that contained a 200 &mu<br />L milk sample, 5 &mu<br />L AFM1 antibody-gold conjugates, and 8 &mu<br />L CAP antibody-gold conjugates<br />the whole assay was completed in 15 min and the results could be interpreted visually or using a reader. This immunostrip has cut-off levels of 0.1 ng/mL and 0.5 ng/mL for AFM1 and CAP, respectively. Analysis of CAP and AFM1 in milk samples revealed that data from the immunostrip test agreed closely with those obtained from ELISA. The two-analyte immunostrip is a rapid way for on-site simultaneous detection of AFM1 and CAP in milk.
- Subjects :
- Analyte
Aflatoxin
chloramphenicol
aflatoxin M1
Health, Toxicology and Mutagenesis
lcsh:Medicine
Food Contamination
Toxicology
01 natural sciences
Article
chemistry.chemical_compound
0404 agricultural biotechnology
gold nanoparticle immunochromatographic strip
antibody
medicine
Animals
Reagent Strips
Immunoassay
Chromatography
Chloramphenicol
010401 analytical chemistry
lcsh:R
Reproducibility of Results
04 agricultural and veterinary sciences
respiratory system
Reference Standards
040401 food science
0104 chemical sciences
Milk sample
Milk
chemistry
Calibration
ELISA
Nitrocellulose
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20726651
- Volume :
- 12
- Issue :
- 637
- Database :
- OpenAIRE
- Journal :
- Toxins
- Accession number :
- edsair.doi.dedup.....4c9cf6403e69d67e35ccee040e04c371