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Chemiluminescent optical fibre genosensor for porcine meat detection
Chemiluminescent optical fibre genosensor for porcine meat detection
- Source :
- Sensors and Actuators B: Chemical. 247:868-874
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- An easy, rapid and sensitive method for the detection of meat species is important to identify adulteration or fraudulent substitution in raw meats with undeclared species. We report herein the development of an optical fibre genosensor able to detect low level of pork (Sus Scrofa) meat in mixtures of minced meat obtained by an optimized and faster DNA extraction protocol. Our approach uses highly specific and sensitive DNA capture and secondary probes designed out of the mitochondrion genome of Sus Scrofa. Once DNA capture probes were immobilized on optical fibres, we demonstrate their putative application as genosensors in detecting Sus Scrofa DNA in purified samples obtained first using a commercial DNA extraction kit and thereafter using our improved extraction method. Furthermore, analysis of beef (Bos taurus) minced meat samples also show that the assay can determine 1% (w/w) of Sus Scrofa DNA. The sensitivity obtained by the optical fibre and the short time required for the results (about 2.5 h) indicate the usefulness of the system. We envision that this genosensor may serve as a diagnostic tool for food sample screening using a technology that is accurate, simple to perform and has the potential to be used in portable format.
- Subjects :
- DNA extraction, Pork meat, Optical fibre genosensor, DNA specific probes
Optical fibre genosensor
01 natural sciences
law.invention
chemistry.chemical_compound
0404 agricultural biotechnology
law
Materials Chemistry
DNA specific probes
Electrical and Electronic Engineering
DNA extraction
Instrumentation
Chemiluminescence
Pork meat
Chromatography
010401 analytical chemistry
Metals and Alloys
food and beverages
Food sample
04 agricultural and veterinary sciences
Condensed Matter Physics
040401 food science
Molecular biology
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
chemistry
Extraction methods
DNA
Subjects
Details
- ISSN :
- 09254005
- Volume :
- 247
- Database :
- OpenAIRE
- Journal :
- Sensors and Actuators B: Chemical
- Accession number :
- edsair.doi.dedup.....4c9641192b1c199036316cc51c15eaad
- Full Text :
- https://doi.org/10.1016/j.snb.2017.03.064