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Growth Factor and Matrix Molecules Preserve Cell Function on Thermally Responsive Culture Surfaces
- Source :
- Tissue Engineering. 5:251-265
- Publication Year :
- 1999
- Publisher :
- Mary Ann Liebert Inc, 1999.
-
Abstract
- Thermally-responsive culture surfaces were designed using copolymers of N-isopropylacrylamide, 4-(aminomethyl)styrene, and acrylic acid. These surfaces contained functional amine and carboxyl groups, which allowed biomolecules to be grafted by amide formation. Epidermal growth factor (EGF), and extracellular matrix (ECM) molecules (collagen type IV, and chondroitin sulfate) were investigated, as surface-grafted biomolecules, for their ability to stimulate cell attachment, proliferation, and function by signaling only from the basal side of cultured cells. Surface analysis of biomolecule-grafted porous inserts showed covalent binding of biomolecules to either amine or carboxyl groups. Multiple attachment to amine and/or carboxyl groups served as cross-linking points that made the polymer hydrogel permanently adherent to the culture surface. Immunofluorescence microscopy techniques gave positive identification of grafted biomolecules. Grafting of EGF improved cell proliferation versus that on nongrafted controls, or controls grafted only with ECM molecules. ECM grafting induced cell attachment on attachment-resistant surfaces. Analysis of trans-epithelial resistance, fluid transport, and polarized g-glutamyl transpeptidase activity indicated that simultaneous grafting of both EGF and ECM produced better polarized cell function than nongrafted controls, or controls grafted with only one type of biomolecule. Covalent grafting of biomolecules did not interfere with cells ability to detach from thermally responsive surfaces upon temperature decrease.
- Subjects :
- Polymers
Surface Properties
Cell Culture Techniques
Biocompatible Materials
Chick Embryo
Matrix (biology)
Extracellular matrix
chemistry.chemical_compound
Epidermal growth factor
Amide
Cell Adhesion
Animals
Chondroitin sulfate
Pigment Epithelium of Eye
Cells, Cultured
Acrylic acid
Extracellular Matrix Proteins
Epidermal Growth Factor
Polyethylene Terephthalates
Cell growth
Chondroitin Sulfates
Temperature
General Engineering
Cell Polarity
gamma-Glutamyltransferase
Amides
chemistry
Biochemistry
Biophysics
Amine gas treating
Collagen
Cell Division
Signal Transduction
Subjects
Details
- ISSN :
- 15578690 and 10763279
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Tissue Engineering
- Accession number :
- edsair.doi.dedup.....4c89c81862da315e6648b7612e9061e9
- Full Text :
- https://doi.org/10.1089/ten.1999.5.251