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Postnatal leptin surge is critical for the transient induction of the developmental beige adipocytes in mice
- Source :
- Am J Physiol Endocrinol Metab
- Publication Year :
- 2020
- Publisher :
- American Physiological Society, 2020.
-
Abstract
- Beige adipocytes have become a promising therapeutic target to combat obesity. Our senior author Dr. B. Xue previously discovered a transient but significant induction of beige adipocytes in mice during early postnatal development, which peaked at postnatal day (P) 20 and then disappeared thereafter. However, the physiological mechanism underlying the transient induction of the developmental beige cells remains mystery. Interestingly, there exists a postnatal surge of leptin in mice at P10 before the appearance of the developmental beige adipocytes. Given the neurotropic effect of leptin during neuronal development and its role in activating the sympathetic nervous system (SNS), we tested the hypothesis that postnatal leptin surge is required for the transient induction of developmental beige adipocytes through sympathetic innervation. Unlike wild-type (WT) mice that were able to acquire the developmentally induced beige adipocytes at P20, ob/ob mice had much less uncoupling protein 1 (UCP1)-positive multilocular cells in inguinal white adipose tissue at the same age. This was consistent with reduced expression of UCP1 mRNA and protein levels in white fat of ob/ob mice. In contrast, daily injection of ob/ob mice with leptin between P8 and P16, mimicking the postnatal leptin surge, largely rescued the ability of these mice to acquire the developmentally induced beige adipocytes at P20, which was associated with enhanced sympathetic nerve innervation assessed by whole mount adipose tissue immunostaining of tyrosine hydroxylase. Our data demonstrate that the postnatal leptin surge is essential for the developmentally induced beige adipocyte formation in mice, possibly through increasing sympathetic nerve innervation.
- Subjects :
- Leptin
Male
0301 basic medicine
Aging
medicine.medical_specialty
Sympathetic nervous system
Sympathetic Nervous System
Tyrosine 3-Monooxygenase
Physiology
Endocrinology, Diabetes and Metabolism
Adipocytes, White
Mice, Obese
Adipose tissue
White adipose tissue
Biology
Mice
03 medical and health sciences
0302 clinical medicine
Physiology (medical)
Internal medicine
medicine
Animals
Adipocytes, Beige
Uncoupling Protein 1
Messenger RNA
Dose-Response Relationship, Drug
Tyrosine hydroxylase
Thermogenin
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Adipose Tissue
Female
030217 neurology & neurosurgery
Immunostaining
Research Article
Subjects
Details
- ISSN :
- 15221555 and 01931849
- Volume :
- 318
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Endocrinology and Metabolism
- Accession number :
- edsair.doi.dedup.....4c85ec690838a565b5fad7b457ee88de