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Inhibitory effect of C-X-C motif chemokine ligand 14 on the osteogenic differentiation of human periodontal ligament cells through transforming growth factor-beta1
- Source :
- Archives of Oral Biology. 115:104733
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Objective This study aimed to determine the expression of chemokine (C-X-C motif) ligand 14 (CXCL14) in pulpal and periodontal cells in vivo and in vitro, and investigate function of CXCL14 and its underlying mechanism in the proliferation and osteogenic differentiation of human periodontal ligament (hPDL) cells. Methods To determine the expression level of CXCL14 in adult rat oral tissues and in hPDL cells after application of biophysical forces, RT-PCR, western blot, and histological analyses were performed. The role of CXCL14 in proliferation and osteogenic differentiation of PDL cells was evaluated by measuring dehydrogenase activity and Alizarin red S staining. Results Strong immunoreactivity against CXCL14 was observed in the PDL tissues and pulpal cells of rat molar, and attenuated apparently by orthodontic biophysical forces. As seen in rat molar, highly expressed CXCL14 was observed in human dental pulp and hPDL cells, and attenuated obviously by biophysical tensile force. CXCL14 expression in hPDL cells was increased in incubation time-dependent manner. Proliferation of hPDL cells was inhibited dramatically by small interfering (si) RNA against CXCL14. Furthermore, dexamethasone-induced osteogenic mineralization was inhibited by recombinant human (rh) CXCL14, and augmented by CXCL14 siRNA. rhCXCL14 increased transforming growth factor-beta1 (TGF- β1) in hPDL cells. Inhibition of the cell proliferation and osteogenic differentiation of hPDL cells by CXCL14 siRNA and rhCXCL14 were restored by rhTGF-β1 and SB431542, respectively. Conclusion These results suggest that CXCL14 may play roles as a growth factor and a negative regulator of osteogenic differentiation by increasing TGF-β1 expression in hPDL cells.
- Subjects :
- 0301 basic medicine
Chemokine
Periodontal Ligament
Chemokine CXCL1
medicine.medical_treatment
Transforming Growth Factor beta1
03 medical and health sciences
0302 clinical medicine
Western blot
Osteogenesis
medicine
Animals
Humans
Periodontal fiber
CXCL14
General Dentistry
Cells, Cultured
medicine.diagnostic_test
biology
Chemistry
Cell growth
Growth factor
Cell Differentiation
030206 dentistry
Cell Biology
General Medicine
In vitro
Rats
Cell biology
030104 developmental biology
Otorhinolaryngology
Transforming Growth Factors
biology.protein
Transforming growth factor
Subjects
Details
- ISSN :
- 00039969
- Volume :
- 115
- Database :
- OpenAIRE
- Journal :
- Archives of Oral Biology
- Accession number :
- edsair.doi.dedup.....4c734d685409c1538aa4e90e0ed9f953