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3D Printed Graphene-PLA Scaffolds Promote Cell Alignment and Differentiation
- Source :
- International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1736, International Journal of Molecular Sciences, Vol 23, Iss 1736, p 1736 (2022)
- Publication Year :
- 2022
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2022.
-
Abstract
- Traumas and chronic damages can hamper the regenerative power of nervous, muscle, and connective tissues. Tissue engineering approaches are promising therapeutic tools, aiming to develop reliable, reproducible, and economically affordable synthetic scaffolds which could provide sufficient biomimetic cues to promote the desired cell behaviour without triggering graft rejection and transplant failure. Here, we used 3D-printing to develop 3D-printed scaffolds based on either PLA or graphene@PLA with a defined pattern. Multiple regeneration strategies require a specific orientation of implanted and recruited cells to perform their function correctly. We tested our scaffolds with induced pluripotent stem cells (iPSC), neuronal-like cells, immortalised fibroblasts and myoblasts. Our results demonstrated that the specific “lines and ridges” 100 µm-scaffold topography is sufficient to promote myoblast and fibroblast cell alignment and orient neurites along with the scaffolds line pattern. Conversely, graphene is critical to promote cells differentiation, as seen by the iPSC commitment to neuroectoderm, and myoblast fusions into multinuclear myotubes achieved by the 100 µm scaffolds containing graphene. This work shows the development of a reliable and economical 3D-printed scaffold with the potential of being used in multiple tissue engineering applications and elucidates how scaffold micro-topography and graphene properties synergistically control cell differentiation.
- Subjects :
- Cell Survival
QH301-705.5
Neurogenesis
Polyesters
Induced Pluripotent Stem Cells
regenerative medicine
G+
scaffold
Catalysis
Cell Fusion
Myoblasts
Inorganic Chemistry
stem cells
Cell Line, Tumor
Neurites
Humans
Physical and Theoretical Chemistry
Biology (General)
Telomerase
Molecular Biology
neuronal differentiation
QD1-999
Spectroscopy
Cell Proliferation
Tissue Scaffolds
graphene, G+, PLA, Grafylon, scaffold, neuronal differentiation, stem cells, 3D printing, regenerative medicine
Organic Chemistry
graphene
PLA
Grafylon
3D printing
Cell Differentiation
General Medicine
Fibroblasts
Computer Science Applications
Chemistry
Printing, Three-Dimensional
Graphite
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1736
- Accession number :
- edsair.doi.dedup.....4c65420b88667290fc993d2be2842270
- Full Text :
- https://doi.org/10.3390/ijms23031736