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Chemiluminescence and chemiluminescence resonance energy transfer (CRET) aptamer sensors using catalytic hemin/G-quadruplexes
- Source :
- ACS Nano, ACS Nano; Vol 5
- Publication Year :
- 2011
-
Abstract
- The incorporation of hemin into the thrombin/G-quadruplex aptamer assembly or into the ATP/G-quadruplex nanostructure yields active DNAzymes that catalyze the generation of chemiluminescence. These catalytic processes enable the detection of thrombin and ATP with detection limits corresponding to 200 pM and 10 μM, respectively. The conjugation of the antithrombin or anti-ATP aptamers to CdSe/ZnS semiconductor quantum dots (QDs) allowed the detection of thrombin or ATP through the luminescence of the QDs that is powered by a chemiluminescence resonance energy-transfer (CRET) process stimulated by the hemin/G-quadruplex/thrombin complex or the hemin/G-quadruplex/ATP nanostructure, in the presence of luminol/H(2)O(2). The advantages of applying the CRET process for the detection of thrombin or ATP, by the resulting hemin/G-quadruplex DNAzyme structures, are reflected by low background signals and the possibility to develop multiplexed aptasensor assays using different sized QDs.
- Subjects :
- Luminescence
Aptamer
Deoxyribozyme
General Physics and Astronomy
02 engineering and technology
010402 general chemistry
G-quadruplex
Photochemistry
7. Clean energy
01 natural sciences
Antithrombins
Catalysis
Luminol
law.invention
chemistry.chemical_compound
Thrombin
Adenosine Triphosphate
law
medicine
heterocyclic compounds
General Materials Science
Chemiluminescence
Chemistry
General Engineering
equipment and supplies
021001 nanoscience & nanotechnology
0104 chemical sciences
G-Quadruplexes
Energy Transfer
Hemin
0210 nano-technology
medicine.drug
Subjects
Details
- ISSN :
- 1936086X
- Volume :
- 5
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- ACS nano
- Accession number :
- edsair.doi.dedup.....4c61ff236587c5b7d0337e0512d8e1da