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Enhanced Oil Removal by a Non-Toxic Biosurfactant Formulation
- Source :
- Energies, Vol 14, Iss 467, p 467 (2021), Energies; Volume 14; Issue 2; Pages: 467
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- In this study, a new formulation of low-cost, biodegradable, and non-toxic biosurfactant by Candida sphaerica UCP 0995 was investigated. The study was conducted in a bioreactor on an industrial waste-based medium, and a central composite rotatable design was used for optimization. The best results, namely a 25.22 mN/m reduction in surface tension, a biosurfactant yield of 10.0 g/L, and a critical micelle concentration of 0.2 g/L, were achieved in 132 h at an agitation speed of 175 rpm and an aeration rate of 1.5 vvm. Compositional and spectroscopic analyses of the purified biosurfactant by chemical methods, Fourier transform infrared spectroscopy, and nuclear magnetic resonance suggested that it is a glycolipid-type biosurfactant, and it showed no cytotoxicity in the MTT assay. The biosurfactant, submitted to different formulation methods as a commercial additive, remained stable for 120 days at room temperature. Tensioactive properties and stability were evaluated at different pH values, temperatures, and salt concentrations. The biosurfactant obtained with all formulation methods demonstrated good stability, with tolerance to wide ranges of pH, temperature and salinity, enabling application under extreme environmental conditions. Bioremediation tests were performed to check the efficacy of the isolated biosurfactant and the selected microbial species in removing oil from soil. The results demonstrated that the biosurfactant produced has promising properties as an agent for the bioremediation of contaminated soil.
- Subjects :
- Control and Optimization
Energy Engineering and Power Technology
010501 environmental sciences
lcsh:Technology
01 natural sciences
petroleum contamination
Industrial waste
industrial waste
Surface tension
03 medical and health sciences
Bioremediation
Bioreactor
Electrical and Electronic Engineering
Fourier transform infrared spectroscopy
Engineering (miscellaneous)
Candida
030304 developmental biology
0105 earth and related environmental sciences
0303 health sciences
Chromatography
lcsh:T
Renewable Energy, Sustainability and the Environment
Chemistry
biosurfactant
Soil contamination
Critical micelle concentration
Yield (chemistry)
Energy (miscellaneous)
Subjects
Details
- ISSN :
- 19961073
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Energies
- Accession number :
- edsair.doi.dedup.....4c57e1a7fef9301c52303c3b2d4c8332
- Full Text :
- https://doi.org/10.3390/en14020467