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Rat kappa-chain J-segment genes: Two recent gene duplications separate rat and mouse
- Source :
- Cell. 29:121-127
- Publication Year :
- 1982
- Publisher :
- Elsevier BV, 1982.
-
Abstract
- We have cloned DNA segments containing the J κ genes from LOUVAIN rat liver, and have determined their nucleotide sequence. Seven readily identifiable J κ -coding regions (six expressible) are evident in the rat, compared with five in the mouse (four expressible). The two additional J segments in the rat appear to be the result of two sequential gene duplications occurring since the divergence of rats and mice. The first involved a homologous but unequal crossing-over in a 14 bp region spanning the 3′ end of the coding region of J1 and J2. The second involved a crossing-over following unequal pairing of the two newly duplicated regions. We propose that the probability of a second duplication was greatly increased following the first as a result of the increased target for unequal pairing (370 bp of good homology versus 27 bp in the original pairing). Comparisons of rat and mouse J genes show a surprisingly high degree of sequence conservation, both inside and outside the coding regions, similar to the pattern we reported previously for the kappa constant-region gene. This provides additional evidence that constraints exist on the nucleotide sequences of these genes independent of the function of the encoded proteins.
- Subjects :
- Biology
General Biochemistry, Genetics and Molecular Biology
Homology (biology)
Immunoglobulin kappa-Chains
Mice
chemistry.chemical_compound
Species Specificity
Gene duplication
Homologous chromosome
Animals
Coding region
Crossing Over, Genetic
Cloning, Molecular
Gene
Genetics
Base Sequence
Nucleic acid sequence
DNA
DNA Restriction Enzymes
Molecular biology
Rats
Genes
chemistry
Immunoglobulin Light Chains
J-segment
Subjects
Details
- ISSN :
- 00928674
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....4c35c9c59e1dca265d5fe99e13e7496b