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Syncytium formation and HIV-1 replication are both accentuated by purified influenza and virus-associated neuraminidase
- Source :
- The Journal of biological chemistry. 277(12)
- Publication Year :
- 2002
-
Abstract
- The degree of sialylation has been shown previously to modulate the process of human immunodeficiency virus type-1 (HIV-1) infection by affecting the interaction between the virus and CD4-expressing target cells. In the present study, we investigated whether HIV-1 replication cycle was affected by neuraminidase (NA) derived from the human influenza (flu) virus. We first demonstrate that the level of HIV-1-mediated syncytium formation was greatly enhanced in the presence of purified flu NA. Pretreatment of established monocytic and lymphocytic cell lines as well as primary mononuclear cells with purified flu NA augmented also the process of virus infection. A comparable up-regulating effect was observed when using several strains of UV-inactivated whole flu virus, thereby suggesting that virus-anchored NA enzymes positively modulate the HIV-1 life cycle. Furthermore, flu NA-mediated positive effect on HIV-1 biology was abrogated with zanamivir, a specific flu NA inhibitor. Our results provide a new model allowing the investigation of the potential benefit of using NA inhibitors in the treatment of HIV-1-infected patients suffering from coinfection with NA-bearing pathogens.
- Subjects :
- CD4-Positive T-Lymphocytes
Time Factors
Ultraviolet Rays
Human immunodeficiency virus (HIV)
Neuraminidase
Cell Communication
Biology
medicine.disease_cause
Biochemistry
Peripheral blood mononuclear cell
Antiviral Agents
Giant Cells
Guanidines
Virus
Monocytes
Microbiology
Cell Line
Jurkat Cells
Zanamivir
medicine
Humans
Lymphocytes
Luciferases
Molecular Biology
Pyrans
chemistry.chemical_classification
Syncytium
Binding Sites
virus diseases
Cell Biology
medicine.disease
Orthomyxoviridae
Virology
Up-Regulation
Enzyme
chemistry
CD4 Antigens
Coinfection
biology.protein
HIV-1
Sialic Acids
Receptors, Virus
medicine.drug
Plasmids
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 277
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....4c1ce2cf4a0f9ea6586ff55020795d7f