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Molecular Mechanism Associated With the Impact of Methane/Oxygen Gas Supply Ratios on Cell Growth of
- Source :
- Frontiers in Bioengineering and Biotechnology, Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
- Publication Year :
- 2020
-
Abstract
- The methane (CH4)/oxygen (O2) gas supply ratios significantly affect the cell growth and metabolic pathways of aerobic obligate methanotrophs. However, few studies have explored the CH4/O2 ratios of the inlet gas, especially for the CH4 concentrations within the explosion range (5∼15% of CH4 in air). This study thoroughly investigated the molecular mechanisms associated with the impact of different CH4/O2 ratios on cell growth of a model type I methanotroph Methylomicrobium buryatense 5GB1 cultured at five different CH4/O2 supply molar ratios from 0.28 to 5.24, corresponding to CH4 content in gas mixture from 5% to 50%, using RNA-Seq transcriptomics approach. In the batch cultivation, the highest growth rate of 0.287 h-1 was achieved when the CH4/O2 supply molar ratio was 0.93 (15% CH4 in air), and it is crucial to keep the availability of carbon and oxygen levels balanced for optimal growth. At this ratio, genes related to methane metabolism, phosphate uptake system, and nitrogen fixation were significantly upregulated. The results indicated that the optimal CH4/O2 ratio prompted cell growth by increasing genes involved in metabolic pathways of carbon, nitrogen and phosphate utilization in M. buryatense 5GB1. Our findings provided an effective gas supply strategy for methanotrophs, which could enhance the production of key intermediates and enzymes to improve the performance of bioconversion processes using CH4 as the only carbon and energy source. This research also helps identify genes associated with the optimal CH4/O2 ratio for balancing energy metabolism and carbon flux, which could be candidate targets for future metabolic engineering practice.
- Subjects :
- 0301 basic medicine
Histology
Methanotroph
Bioconversion
lcsh:Biotechnology
Biomedical Engineering
chemistry.chemical_element
Bioengineering
02 engineering and technology
Oxygen
Methane
Metabolic engineering
03 medical and health sciences
chemistry.chemical_compound
transcriptomics
lcsh:TP248.13-248.65
Food science
methanotroph
CH4/O2 gas supply ratio
Original Research
Chemistry
Bioengineering and Biotechnology
methane metabolism
021001 nanoscience & nanotechnology
Phosphate
Metabolic pathway
030104 developmental biology
nitrogen fixation
0210 nano-technology
Energy source
Biotechnology
Methylomicrobium buryatense
Subjects
Details
- ISSN :
- 22964185
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Frontiers in bioengineering and biotechnology
- Accession number :
- edsair.doi.dedup.....4c18b83fbf481a8a24202c8e94373463