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Human testis in organotypic culture: application for basic or clinical research
- Source :
- Human Reproduction, Human Reproduction, 2006, 21 (6), pp.1564-1575, Human Reproduction, Oxford University Press (OUP), 2006, 21 (6), pp.1564-75. ⟨10.1093/humrep/del018⟩, Human Reproduction, Oxford University Press (OUP), 2006, 21 (6), pp.1564-1575, Human Reproduction, 2006, 21 (6), pp.1564-75. ⟨10.1093/humrep/del018⟩
- Publication Year :
- 2006
- Publisher :
- Oxford University Press (OUP), 2006.
-
Abstract
- International audience; BACKGROUND: Over recent decades, recurring efforts have been devoted to developing testicular cell or tissue cultures for basic and clinical research. However, there remains much confusion, particularly concerning the fate of human germ cells in culture. OBJECTIVE: To reassess the status of human testicular cell types as well as the ability of germ cells to divide and differentiate in organotypic culture. METHODS: Human testicular fragments were maintained for 2 weeks in culture. The viability and functionality of testicular cells were assessed using light and electronic microscopy, apoptotic cell labelling, 5-bromo-2'-deoxyuridine (BrdU) incorporation, immunohistochemistry and quantitative PCR against specific cell markers. RESULTS: A gradual loss of meiotic and post-meiotic germ cells occurred throughout the culture period, irrespective of the presence of gonadotrophins. However, all germ cell types remained traceable for up to 16 days, some still dividing and differentiating at a rate compatible with the in vivo situation. Good maintenance of the general architecture of the explants associated with clearly quantifiable levels of several somatic cell markers was observed. CONCLUSION: Although this culture model is clearly unsuitable for preparing germ cells for therapeutic purposes, it does represent a most valuable tool for testing the effects of biological and chemical agents on testicular tissue.
- Subjects :
- Male
Pathology
Time Factors
Somatic cell
[SDV]Life Sciences [q-bio]
Cellular differentiation
Cell
Testicle
Tissue culture
0302 clinical medicine
Testis
Cyclic AMP
TISSU TESTICULAIRE
MESH: In Situ Nick-End Labeling
MESH: Cyclic AMP
ComputingMilieux_MISCELLANEOUS
MESH: Bromodeoxyuridine
0303 health sciences
030219 obstetrics & reproductive medicine
MESH: Testis
Rehabilitation
Obstetrics and Gynecology
Cell Differentiation
Immunohistochemistry
[SDV] Life Sciences [q-bio]
Meiosis
medicine.anatomical_structure
Germ cell
MESH: Cell Differentiation
endocrine system
medicine.medical_specialty
Cell type
HUMAIN
DNA Fragmentation
[INFO] Computer Science [cs]
Biology
Andrology
03 medical and health sciences
Organ Culture Techniques
In Situ Nick-End Labeling
medicine
MESH: DNA Fragmentation
Humans
[INFO]Computer Science [cs]
human
030304 developmental biology
MESH: Humans
CULTURE ORGANOTYPIQUE
MESH: Time Factors
MESH: Immunohistochemistry
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
MESH: Organ Culture Techniques
MESH: Male
MESH: Meiosis
MESH: Germ Cells
Germ Cells
organotypic culture
Bromodeoxyuridine
Reproductive Medicine
MESH: Gonadotropins
CULTURE DE CELLULE
Gonadotropins
Explant culture
Subjects
Details
- ISSN :
- 14602350 and 02681161
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Human Reproduction
- Accession number :
- edsair.doi.dedup.....4c07fe5537566a64663046885de1579e
- Full Text :
- https://doi.org/10.1093/humrep/del018