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Human testis in organotypic culture: application for basic or clinical research

Authors :
Jean-Jacques Patard
Nathalie Dejucq-Rainsford
Bernard Jégou
B. Delaleu
V. Roulet
Ap Satie
Hélène Denis
C. Staub
A. Le Tortorec
Groupe d'Etude de la Reproduction Chez l'Homme et les Mammiferes (GERHM)
Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Université de Rennes (UR)
Physiologie de la reproduction et des comportements [Nouzilly] (PRC)
Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
CHU Pontchaillou [Rennes]
ProdInra, Migration
Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1)
Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)
Service d'urologie [Rennes] = Urology [Rennes]
Hôpital Pontchaillou-CHU Pontchaillou [Rennes]
INSERM
Ministère de l'Enseignement Supérieur et de la Recherche
ANRS
Sidaction
Région Bretagne
Université de Rennes 1 (UR1)
Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Université de Rennes (UNIV-RENNES)
Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Source :
Human Reproduction, Human Reproduction, 2006, 21 (6), pp.1564-1575, Human Reproduction, Oxford University Press (OUP), 2006, 21 (6), pp.1564-75. ⟨10.1093/humrep/del018⟩, Human Reproduction, Oxford University Press (OUP), 2006, 21 (6), pp.1564-1575, Human Reproduction, 2006, 21 (6), pp.1564-75. ⟨10.1093/humrep/del018⟩
Publication Year :
2006
Publisher :
Oxford University Press (OUP), 2006.

Abstract

International audience; BACKGROUND: Over recent decades, recurring efforts have been devoted to developing testicular cell or tissue cultures for basic and clinical research. However, there remains much confusion, particularly concerning the fate of human germ cells in culture. OBJECTIVE: To reassess the status of human testicular cell types as well as the ability of germ cells to divide and differentiate in organotypic culture. METHODS: Human testicular fragments were maintained for 2 weeks in culture. The viability and functionality of testicular cells were assessed using light and electronic microscopy, apoptotic cell labelling, 5-bromo-2'-deoxyuridine (BrdU) incorporation, immunohistochemistry and quantitative PCR against specific cell markers. RESULTS: A gradual loss of meiotic and post-meiotic germ cells occurred throughout the culture period, irrespective of the presence of gonadotrophins. However, all germ cell types remained traceable for up to 16 days, some still dividing and differentiating at a rate compatible with the in vivo situation. Good maintenance of the general architecture of the explants associated with clearly quantifiable levels of several somatic cell markers was observed. CONCLUSION: Although this culture model is clearly unsuitable for preparing germ cells for therapeutic purposes, it does represent a most valuable tool for testing the effects of biological and chemical agents on testicular tissue.

Details

ISSN :
14602350 and 02681161
Volume :
21
Database :
OpenAIRE
Journal :
Human Reproduction
Accession number :
edsair.doi.dedup.....4c07fe5537566a64663046885de1579e
Full Text :
https://doi.org/10.1093/humrep/del018