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Efficacy of rhNGF-loaded amniotic membrane transplantation for rabbit corneal epithelial and nerve regeneration
- Source :
- Int J Ophthalmol, International Journal of Ophthalmology, Vol 14, Iss 11, Pp 1653-1659 (2021)
- Publication Year :
- 2021
- Publisher :
- International Journal of Ophthalmology Press, 2021.
-
Abstract
- AIM: To evaluate the efficacy of recombinant human nerve growth factor-loaded amniotic membrane (rhNGF-AM) on corneal epithelial and nerve regeneration in rabbit model. METHODS: Freshly prepared human amniotic membrane (AM) were immersed into PBS buffer containing 100 or 500 μg/mL rhNGF for 15, 30, and 60min at 4℃. The in vitro release kinetics of rhNGF was measured with ELISA. For in vivo evaluation, the AM were immersed with 500 μg/mL rhNGF for 30min. Fifty-seven rabbits were selected to establish corneal epithelial defect model. In addition to the 19 rabbits in control group, 38 rabbits received AM transplantation with or without rhNGF after the removal of central epithelium. Corneal epithelial defect area, sub-epithelial nerve fiber density, corneal sensitivity, rhNGF contents in resident AM and corneas were measured after the surgery. RESULTS: rhNGF was sustained release from the AM within 14d in vitro, with the positive correlation with initial immersion concentration. The immersion of AM in 500 μg/mL rhNGF for 30min achieved the most stable release within 14d. After transplantation in rabbit cornea, a high concentration of rhNGF in resident rhNGF-AM and cornea was maintained within 8d. Corneal epithelial healing, nerve fiber regeneration and the recovery of corneal sensitivity were significantly accelerated after the rhNGF-AM transplantation when compared to simple AM transplantation (all P
- Subjects :
- corneal epithelium
medicine.medical_specialty
amniotic membrane
drug-loaded
business.industry
Regeneration (biology)
Nerve fiber
RE1-994
In vitro
Epithelium
eye diseases
Transplantation
Ophthalmology
recombinant human nerve growth factor
medicine.anatomical_structure
Basic Research
In vivo
Cornea
Medicine
sense organs
nerve regeneration
business
Corneal epithelium
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Int J Ophthalmol, International Journal of Ophthalmology, Vol 14, Iss 11, Pp 1653-1659 (2021)
- Accession number :
- edsair.doi.dedup.....4bfe787b431113594a228dcfa8b12ae0