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Carboxymethyl fenugreek galactomannan-g-poly(N-isopropylacrylamide-co-N,N′-methylene-bis-acrylamide)-clay based pH/temperature-responsive nanocomposites as drug-carriers
- Source :
- Materials Science and Engineering: C. 110:110628
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The current study dealt with the synthesis and characterization of carboxymethyl fenugreek galactomannang-g-poly(N-isopropylacrylamide-co-N,N′-methylene-bis-acrylamide)-bentonite [CFG-g-P(NIPA-co-MBA)-BEN] based nanocomposites (NCs) as erlotinib (ERL)-delivery devices for lung cancer cells to suppress excessive cell proliferation. The blank NCs exhibited outstanding biodegradability and pH/temperature-dependent swelling profiles, which were significantly influenced by their BEN contents (0–20%). The molar mass ( M ¯ c) between the crosslinks of these NCs was declined with temperature. The composite architecture of these scaffolds was confirmed by XRD, FTIR, TGA, DSC and SEM analyses. The corresponding ERL-loaded matrices (F-1-F-3) portrayed outstanding drug encapsulation efficiency (DEE, 93–100%) with zeta potential between −8 and −16 mV and diameter between 615 and 1258 nm. These formulations demonstrated sustained ERL elution profiles (Q8h, 62–98%) with an initial burst release of drug. The drug dissolution pattern of the optimized matrices (F-3) obeyed first-order kinetic model and was driven by Fickian diffusion. The mucin adsorption behavior of F-3 was best fitted to Freudlich isotherms. The ERL-loaded formulation suppressed A549 cell proliferation and promoted apoptosis to a greater extent than the pristine drug, as detected by cellular uptake analysis, MTT cytotoxicity test and AO/EB staining assay.
- Subjects :
- Hot Temperature
Lung Neoplasms
Materials science
Apoptosis
Bioengineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Mannans
Biomaterials
chemistry.chemical_compound
medicine
Zeta potential
Humans
Dissolution testing
Cell Proliferation
Acrylamides
Drug Carriers
Molar mass
Galactose
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
0104 chemical sciences
Models, Chemical
chemistry
A549 Cells
Mechanics of Materials
Delayed-Action Preparations
Acrylamide
Drug delivery
Poly(N-isopropylacrylamide)
Swelling
medicine.symptom
0210 nano-technology
Drug carrier
Nuclear chemistry
Subjects
Details
- ISSN :
- 09284931
- Volume :
- 110
- Database :
- OpenAIRE
- Journal :
- Materials Science and Engineering: C
- Accession number :
- edsair.doi.dedup.....4bd54918618271a03762a218aa3486b1