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Triple-Color Super-Resolution Imaging of Live Cells: Resolving Submicroscopic Receptor Organization in the Plasma Membrane
- Source :
- Angewandte Chemie International Edition; Vol 51, Angewandte Chemie International Edition
- Publication Year :
- 2012
- Publisher :
- WILEY-V C H VERLAG GMBH, 2012.
-
Abstract
- In living color: efficient intracellular covalent labeling of proteins with a photoswitchable dye using the HaloTag for dSTORM super-resolution imaging in live cells is described. The dynamics of cellular nanostructures at the plasma membrane were monitored with a time resolution of a few seconds. In combination with dual-color FPALM imaging, submicroscopic receptor organization within the context of the membrane skeleton was resolved.
- Subjects :
- Green Fluorescent Proteins
Context (language use)
010402 general chemistry
Transfection
01 natural sciences
Catalysis
Protein–protein interaction
03 medical and health sciences
Microscopy
Humans
Photoactivated localization microscopy
Cytoskeleton
Fluorescent Dyes
030304 developmental biology
0303 health sciences
Chemistry
010405 organic chemistry
Cell Membrane
General Chemistry
General Medicine
Fluorescence
Actins
Nanostructures
0104 chemical sciences
Membrane
Biochemistry
Membrane protein
Microscopy, Fluorescence
Biophysics
Intracellular
HeLa Cells
Subjects
Details
- Language :
- English
- ISSN :
- 14337851
- Volume :
- 51
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Angewandte Chemie International Edition
- Accession number :
- edsair.doi.dedup.....4bccb5cb83b0643369eee664b2213ae4
- Full Text :
- https://doi.org/10.1002/anie.201200853