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Arylsulfatase multiplicity in Proteus rettgeri
- Source :
- Canadian Journal of Microbiology. 16:1109-1115
- Publication Year :
- 1970
- Publisher :
- Canadian Science Publishing, 1970.
-
Abstract
- Multiple electrophoretic species of arylsulfatase were demonstrated in sonicates of Proteus rettgeri by paper electrophoresis. Subsequent treatment of the sonicates with streptomycin sulfate and DEAE-cellulose chromatography resolved enzyme activity into four fractions. Electrophoretic examination of each chromatographic fraction revealed sulfatase heterogeneity comparable to that of the initial extract. These electrophoretic components differed in their general response to a number of arylsulfatase inhibitors. The results are discussed in relation to the finding of multiple species of arylsulfatase in other biological systems.
- Subjects :
- Electrophoresis
Paper
Immunology
Streptomycin Sulfate
Catechols
Paper electrophoresis
Acetates
Sodium Chloride
Biology
Vibration
Applied Microbiology and Biotechnology
Microbiology
Chromatography, DEAE-Cellulose
Phosphates
Nitrophenols
Genetics
Ultrasonics
Molecular Biology
Cyanides
Chromatography
Cell-Free System
Sulfates
Sulfatase
Sodium
General Medicine
Proteus
Multiple species
Enzyme assay
Biochemistry
Spectrophotometry
Streptomycin
biology.protein
Sulfatases
Proteus rettgeri
Arylsulfatase
Subjects
Details
- ISSN :
- 14803275 and 00084166
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....4bc2e38c0238c82a147c8c6d82614e16