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An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain
- Source :
- Journal of Neuroscience Methods. 160:223-230
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- The amino acid neurotransmitters γ-aminobutyric acid (GABA) and glutamate have been implicated in mood disorders and high performance liquid chromatography (HPLC) is an important method in their quantitation. This paper describes a method which employs either electrochemical or fluorescent detection of the amino acid derivatives formed by a reaction with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide ions. Elution is based on an isocratic protocol and is achieved in 20 min or less. The method is reproducible, shows excellent linearity and generates derivatives which are stable for up to 16 h. Successful application of the method to a maternal deprivation study has identified the hippocampus and the brainstem as possible sites of long-term alteration in this paradigm.
- Subjects :
- Male
Cyanide
Glutamic Acid
Naphthalenes
Hippocampus
High-performance liquid chromatography
Rats, Sprague-Dawley
chemistry.chemical_compound
Animals
Chromatography, High Pressure Liquid
gamma-Aminobutyric Acid
Brain Chemistry
chemistry.chemical_classification
Chromatography
Behavior, Animal
Elution
Maternal Deprivation
General Neuroscience
Glutamate receptor
Brain
Neurochemistry
Fluorescence
Rats
Amino acid
chemistry
Brain Stem
Subjects
Details
- ISSN :
- 01650270
- Volume :
- 160
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroscience Methods
- Accession number :
- edsair.doi.dedup.....4bc0c783dc20458e52387d69709a6ca8