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A truncated fragment of Ov-ASP-1 consisting of the core pathogenesis-related-1 (PR-1) domain maintains adjuvanticity as the full-length protein
- Source :
- Vaccine
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Highlights • Ov-ASP-1, an onchocerca volvulus protein, has good adjuvanticity for protein antigens. • A truncated Ov-ASP-1 (ASPPR) maintains adjuvanticity as the full-length of Ov-ASP-1. • ASPPR augments humoral and cellular immune responses elicited by protein antigens. • ASPPR has potential to be further developed as a novel adjuvant for human use.<br />The Onchocerca volvulus activation-associated secreted protein-1 (Ov-ASP-1) has good adjuvanticity for a variety of antigens and vaccines, probably due to its ability activate antigen-processing cells (APCs). However, the functional domain of Ov-ASP-1 as an adjuvant is not clearly defined. Based on the structural prediction of this protein family, we constructed a 16-kDa recombinant protein of Ov-ASP-1 that contains only the core pathogenesis-related-1 (PR-1) domain (residues 10–153), designated ASPPR. We found that ASPPR exhibits adjuvanticity similar to that of the full-length Ov-ASP-1 (residues 10–220) for various antigens, including ovalbumin (OVA), HBsAg protein antigen, and the HIV peptide 5 (Pep5) antigen, but it is more suitable for vaccine design in ASPPR-antigen fusion proteins, and more stable in PBS than Ov-ASP-1 stored at −70 °C. These results suggest that ASPPR might be the functional region of Ov-ASP-1 as an adjuvant, and therefore could be developed as an adjuvant for human use.
- Subjects :
- Helminth protein
Peptide
law.invention
CAP, CRISPS, Ag 5 and PR-1
Mice
law
Pathogenesis-related-1 family
Adjuvant
chemistry.chemical_classification
Immunity, Cellular
ASPPR, PR-1 domain of Ov-ASP-1
biology
Helminth Proteins
Infectious Diseases
Recombinant DNA
Molecular Medicine
Female
Protein family
Ovalbumin
Antibodies, Helminth
CRISPS, cysteine-rich secretory protein
Article
OVA, ovalbumin
Interferon-gamma
FBS, fetal bovine serum
Th1/Th2 immune responses
Adjuvants, Immunologic
Antigen
Adjuvanticity
parasitic diseases
Ov-ASP-1
Animals
Humans
Protein Interaction Domains and Motifs
Hepatitis B Antibodies
Hepatitis B Surface Antigens
General Veterinary
General Immunology and Microbiology
Public Health, Environmental and Occupational Health
Ov-ASP-1, Onchocerca volvulus activation-associated secreted protein-1
PR-1, pathogenesis-related 1
Fusion protein
Virology
Molecular biology
Immunity, Humoral
Onchocerca volvulus
chemistry
HIV-1
biology.protein
Immunization
Ag 5, antigen 5
Interleukin-4
HBsAg, Hepatitis B virus surface antigen
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....4bbbcdc5f38c9aed980f6a512a7314b9