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Synthesis, characterization and in vitro study of biocompatible cinnamaldehyde functionalized magnetite nanoparticles (CPGF Nps) for hyperthermia and drug delivery applications in breast cancer
- Source :
- PLoS ONE, Vol 9, Iss 9, p e107315 (2014), PLoS ONE
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- Cinnamaldehyde, the bioactive component of the spice cinnamon, and its derivatives have been shown to possess anti-cancer activity against various cancer cell lines. However, its hydrophobic nature invites attention for efficient drug delivery systems that would enhance the bioavailability of cinnamaldehyde without affecting its bioactivity. Here, we report the synthesis of stable aqueous suspension of cinnamaldehyde tagged Fe3O4 nanoparticles capped with glycine and pluronic polymer (CPGF NPs) for their potential application in drug delivery and hyperthermia in breast cancer. The monodispersed superparamagnetic NPs had an average particulate size of ∼ 20 nm. TGA data revealed the drug payload of ∼ 18%. Compared to the free cinnamaldehyde, CPGF NPs reduced the viability of breast cancer cell lines, MCF7 and MDAMB231, at lower doses of cinnamaldehyde suggesting its increased bioavailability and in turn its therapeutic efficacy in the cells. Interestingly, the NPs were non-toxic to the non-cancerous HEK293 and MCF10A cell lines compared to the free cinnamaldehyde. The novelty of CPGF nanoparticulate system was that it could induce cytotoxicity in both ER/PR positive/Her2 negative (MCF7) and ER/PR negative/Her2 negative (MDAMB231) breast cancer cells, the latter being insensitive to most of the chemotherapeutic drugs. The NPs decreased the growth of the breast cancer cells in a dose-dependent manner and altered their migration through reduction in MMP-2 expression. CPGF NPs also decreased the expression of VEGF, an important oncomarker of tumor angiogenesis. They induced apoptosis in breast cancer cells through loss of mitochondrial membrane potential and activation of caspase-3. Interestingly, upon exposure to the radiofrequency waves, the NPs heated up to 41.6 °C within 1 min, suggesting their promise as a magnetic hyperthermia agent. All these findings indicate that CPGF NPs prove to be potential nano-chemotherapeutic agents in breast cancer.
- Subjects :
- Cancer Treatment
lcsh:Medicine
Biocompatible Materials
Cinnamaldehyde
chemistry.chemical_compound
Drug Stability
Cell Movement
Basic Cancer Research
Medicine and Health Sciences
Materials Chemistry
Medicine
Acrolein
Cytotoxicity
Magnetite Nanoparticles
lcsh:Science
Membrane Potential, Mitochondrial
Drug Carriers
Multidisciplinary
Cancer Drug Discovery
Physics
Oncology
Drug delivery
Physical Sciences
Interdisciplinary Physics
MCF-7 Cells
Female
Drug carrier
Research Article
Biotechnology
Cell Survival
Herbal Medicine
Materials Science
Glycine
Antineoplastic Agents
Breast Neoplasms
Poloxamer
Chemoprevention
Biomaterials
Inhibitory Concentration 50
Complementary and Alternative Medicine
Humans
Cell Proliferation
Cell growth
business.industry
lcsh:R
technology, industry, and agriculture
Biology and Life Sciences
Hyperthermia, Induced
Kinetics
Magnetic hyperthermia
HEK293 Cells
chemistry
Apoptosis
Cancer research
lcsh:Q
Drug Screening Assays, Antitumor
business
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....4b98f16227253723ca779281f9ee6ec5