Back to Search
Start Over
Polymorphisms of melatonin receptor genes and their associations with egg production traits in Shaoxing duck
- Source :
- Asian-Australasian Journal of Animal Sciences, Asian-Australasian Journal of Animal Sciences, Vol 31, Iss 10, Pp 1535-1541 (2018)
- Publication Year :
- 2017
-
Abstract
- Objective In birds, three types of melatonin receptors (MTNR1A, MTNR1B, and MTNR1C) have been cloned. Previous researches have showed that three melatonin receptors played an essential role in reproduction and ovarian physiology. However, the association of polymorphisms of the three receptors with duck reproduction traits and egg quality traits is still unknown. In this test, we chose MTNR1A, MTNR1B, and MTNR1C as candidate genes to detect novel sequence polymorphism and analyze their association with egg production traits in Shaoxing duck, and detected their mRNA expression level in ovaries. Methods In this study, a total of 785 duck blood samples were collected to investigate the association of melatonin receptor genes with egg production traits and egg quality traits using a direct sequencing method. And 6 ducks representing two groups (3 of each) according to the age at first eggs (at 128 days of age or after 150 days of age) were carefully selected for quantitative real-time polymerase chain reaction. Results Seven novel polymorphisms (MTNR1A: g. 268C>T, MTNR1B: g. 41C>T, and g. 161T>C, MTNR1C: g. 10C>T, g. 24A>G, g. 108C>T, g. 363 T>C) were detected. The single nucleotide polymorphism (SNP) of MTNR1A (g. 268C>T) was significantly linked with the age at first egg (pT and egg production traits: total egg numbers at 34 weeks old of age and age at first egg. In addition, the mRNA expression level of MTNR1A in ovary was significantly higher in late-mature group than in early-mature group, while MTNR1C showed a contrary tendency (p
- Subjects :
- 0301 basic medicine
Candidate gene
medicine.medical_specialty
lcsh:Animal biochemistry
Single-nucleotide polymorphism
Biology
Single Nucleotide Polymorphism Polymorphism
Melatonin receptor
Article
law.invention
Andrology
Melatonin
03 medical and health sciences
law
Molecular genetics
medicine
Receptor
lcsh:QP501-801
Gene
Polymerase chain reaction
lcsh:SF1-1100
MTNR1A
Egg Production Traits
MTNR1B
MTNR1C
Animal Breeding and Genetics
030104 developmental biology
Animal Science and Zoology
lcsh:Animal culture
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 10112367
- Volume :
- 31
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Asian-Australasian journal of animal sciences
- Accession number :
- edsair.doi.dedup.....4b8f3b0fc5c16f00abfc70e0a3bcc40e