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Label-free photoacoustic nanoscopy
- Publication Year :
- 2014
- Publisher :
- Society of Photo-Optical Instrumentation Engineers, 2014.
-
Abstract
- Super-resolution microscopy techniques - capable of overcoming the diffraction limit of light - have opened new opportunities to explore subcellular structures and dynamics not resolvable in conventional far-field microscopy. However, relying on staining with exogenous fluorescent markers, these techniques can sometimes introduce undesired artifacts to the image, mainly due to large tagging agent sizes and insufficient or variable labeling densities. By contrast, the use of endogenous pigments allows imaging of the intrinsic structures of biological samples with unaltered molecular constituents. Here, we report label-free photoacoustic (PA) nanoscopy, which is exquisitely sensitive to optical absorption, with an 88 nm resolution. At each scanning position, multiple PA signals are successively excited with increasing laser pulse energy. Because of optical saturation or nonlinear thermal expansion, the PA amplitude depends on the nonlinear incident optical fluence. The high-order dependence, quantified by polynomial fitting, provides super-resolution imaging with optical sectioning. PA nanoscopy is capable of super-resolution imaging of either fluorescent or nonfluorescent molecules.
- Subjects :
- Materials science
Optical sectioning
Research Papers: Imaging
Biomedical Engineering
Melanoma, Experimental
Sensitivity and Specificity
law.invention
Biomaterials
Photoacoustic Techniques
Mice
Optics
law
Microscopy
Animals
Nanotechnology
Photoacoustic spectroscopy
Staining and Labeling
Super-resolution microscopy
business.industry
Lasers
Resolution (electron density)
Reproducibility of Results
Equipment Design
Fibroblasts
Laser
Image Enhancement
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Molecular Imaging
Equipment Failure Analysis
NIH 3T3 Cells
Molecular imaging
business
Subcellular Fractions
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....4b8d60ad5d70192b2a5662cb51acd80e