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Role of Cortico-Cancellous Heterologous Bone in Human Periodontal Ligament Stem Cell Xeno-Free Culture Studied by Synchrotron Radiation Phase-Contrast Microtomography
- Source :
- International Journal of Molecular Sciences, Vol 18, Iss 2, p 364 (2017), International Journal of Molecular Sciences; Volume 18; Issue 2; Pages: 364, International Journal of Molecular Sciences
- Publication Year :
- 2017
- Publisher :
- MDPI AG, 2017.
-
Abstract
- This study was designed to quantitatively demonstrate via three-dimensional (3D) images, through the Synchrotron Radiation Phase-Contrast Microtomography (SR-PhC-MicroCT), the osteoinductive properties of a cortico-cancellous scaffold (Osteobiol Dual Block-DB) cultured with human Periodontal Ligament Stem Cells (hPDLSCs) in xeno-free media. In vitro cultures of hPDLSCs, obtained from alveolar crest and horizontal fibers of the periodontal ligament, were seeded onto DB scaffolds and cultured in xeno-free media for three weeks. 3D images were obtained by SR-PhC-microCT after one and three weeks from culture beginning. MicroCT data were successively processed with a phase-retrieval algorithm based on the Transport of Intensity Equation (TIE). The chosen experimental method, previously demonstratively applied for the 3D characterization of the same constructs in not xeno-free media, quantitatively monitored also in this case the early stages of bone formation in basal and differentiating conditions. Interestingly, it quantitatively showed in the xeno-free environment a significant acceleration of the mineralization process, regardless of the culture (basal/differentiating) medium. This work showed in 3D that the DB guides the osteogenic differentiation of hPDLSCs in xeno-free cultures, in agreement with 2D observations and functional studies previously performed by some of the authors. Indeed, here we fully proved in 3D that expanded hPDLSCs, using xeno-free media formulation, not only provide the basis for Good Manufacturing Practice (preserving the stem cells' morphological features and their ability to differentiate into mesenchymal lineage) but have to be considered, combined to DB scaffolds, as interesting candidates for potential clinical use in new custom made tissue-engineered constructs.
- Subjects :
- Adult
0301 basic medicine
Scaffold
Pathology
medicine.medical_specialty
Periodontal ligament stem cells
Periodontal Ligament
Cell Culture Techniques
Heterologous
Article
Catalysis
osteogenesis
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
Periodontal fiber
Microscopy, Phase-Contrast
periodontal ligament stem cells
phase-contrast microtomography
biomaterial
xeno-free medium
Physical and Theoretical Chemistry
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
Tissue Engineering
Tissue Scaffolds
Chemistry
Stem Cells
Organic Chemistry
Mesenchymal stem cell
Cell Differentiation
030206 dentistry
General Medicine
Xeno free
In vitro
Computer Science Applications
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Stem cell
Synchrotrons
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 18
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....4b80089e553a4e98a31f3c85c8c06a2d