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Increased expression of the CD80 accessory molecule by alveolar macrophages in asthmatic subjects and its functional involvement in allergen presentation to autologous TH2 lymphocytes
- Source :
- Journal of Allergy and Clinical Immunology. 103:1136-1142
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- Background: Alveolar macrophages (AMs) are more efficient antigen-presenting cells in allergic individuals than in nonatopic subjects. Objective: We studied whether this difference may be correlated to increased expression of membrane costimulatory molecules, such as the B7 molecules (CD80 and CD86). Methods: Eleven subjects with allergic asthma sensitized to Dermatophagoides pteronyssinus and 5 healthy nonatopic volunteers underwent bronchoalveolar lavage, and the costimulatory molecule expression on AMs was evaluated. Peripheral blood T cells, either freshly isolated or as established D pteronyssinus -specific cell lines, were cultured with autologous monocytes or AMs as antigen-presenting cells. In vitro allergen-induced proliferation and cytokine production were evaluated in the presence of B7-blocking reagents. Results: Allergic individuals had a significantly higher proportion of AMs expressing the CD80 molecule than control subjects (28.5% ± 14.8% vs 1.4% ± 1.2%; P P > .1). In a large proportion of the asthmatic subjects we studied, AMs were presenting soluble antigens (tetanus toxoid and streptolysin-O) to freshly isolated T cells more efficiently than AMs from nonatopic control subjects. Finally, both T-cell proliferation and cytokine production of D pteronyssinus- specific established T-cell lines were inhibited by a CD80-blocking antibody in a dose-dependent manner. Conclusion: Costimulation by means of CD80 expressed by AMs is probably involved in the amplification of the allergen-specific T-lymphocyte response in the airways of asthmatic subjects. (J Allergy Clin Immunol 1999;103:1136-42.)
- Subjects :
- Adult
Male
Cellular immunity
Adolescent
medicine.medical_treatment
Immunology
Antigen-Presenting Cells
Th2 Cells
Antigen
Antigens, CD
Macrophages, Alveolar
Humans
Immunology and Allergy
Medicine
Antigen-presenting cell
Interleukin 5
CD86
Membrane Glycoproteins
business.industry
Monocyte
Asthma
Respiratory Function Tests
medicine.anatomical_structure
Cytokine
B7-1 Antigen
Cytokines
Female
B7-2 Antigen
Interleukin-4
Interleukin-5
business
Bronchoalveolar Lavage Fluid
CD80
Subjects
Details
- ISSN :
- 00916749
- Volume :
- 103
- Database :
- OpenAIRE
- Journal :
- Journal of Allergy and Clinical Immunology
- Accession number :
- edsair.doi.dedup.....4b77d427dadcb6db3364fb7dee54d4c1