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Interactions between hen egg-white lysozyme, PEG2,000, and PLA50 at the air–water interface
- Source :
- Colloids and Surfaces B: Biointerfaces. 42:97-106
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- In this paper, we compared the efficiency of polymer films, made of a poly(ethylene glycol) (PEG2,000)/poly( d , l -lactide) (PLA50) mixture, or a PEG2,000-PLA50 copolymer, to prevent adsorption of a model protein, the hen egg-white lysozyme (HEWL), at the air–water interface. This was achieved by analyzing the surface pressure/surface area curves, and the X-ray reflectivity data of the polymer films spread on a Langmuir trough, obtained in absence or in presence of the protein. For both the mixture and the copolymer, the amount of protein adsorbed at the air–water interface decreases when the density of the polymer surface coverage increases. It was shown that even in a condensed state, the polymer film made by the mixture can not totally prevent HEWL molecules to adsorb and penetrate the polymer mixed film, but however, protein molecules would not be directly exposed to the more hydrophobic phase, i.e. the air phase. It was also shown that the configuration adopted by the copolymer at the interface in its condensed state would prevent adsorption of HEWL molecules for several hours; this would be due in particular to the presence of PEG segments in the interfacial film.
- Subjects :
- Time Factors
Materials science
Polymers
Polyesters
Biophysics
Microscopy, Atomic Force
Surface pressure
Biophysical Phenomena
Polyethylene Glycols
chemistry.chemical_compound
Colloid and Surface Chemistry
Adsorption
Egg White
Phase (matter)
Polymer chemistry
Pressure
Copolymer
Animals
Colloids
Lactic Acid
Physical and Theoretical Chemistry
chemistry.chemical_classification
Air
X-Rays
Temperature
Proteins
Water
Surfaces and Interfaces
General Medicine
Polymer
X-ray reflectivity
Kinetics
Chemical engineering
chemistry
Muramidase
Lysozyme
Chickens
Ethylene glycol
Biotechnology
Subjects
Details
- ISSN :
- 09277765
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Colloids and Surfaces B: Biointerfaces
- Accession number :
- edsair.doi.dedup.....4b5fdb47f910ae1c7d573ee950884b0f