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Trinitrobenzene sulfonic acid-induced intestinal injury in neonatal mice activates transcriptional networks similar to those seen in human necrotizing enterocolitis
- Source :
- Pediatric research
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- We have shown previously that enteral administration of 2, 4, 6-trinitrobenzene sulfonic acid in 10-d-old C57BL/6 pups produces an acute necrotizing enterocolitis with histopathological and inflammatory changes similar to human necrotizing enterocolitis (NEC). To determine whether murine neonatal 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-mediated intestinal injury could be used as a NEC model, we compared gene expression profiles of TNBS-mediated intestinal injury and NEC.Whole-genome microarray analysis was performed on proximal colon from control and TNBS-treated pups (n = 8/group). For comparison, we downloaded human microarray data of NEC (n = 5) and surgical control (n = 4) from a public database. Data were analyzed using the software programs Partek Genomics Suite and Ingenuity Pathway Analysis.We detected extensive changes in gene expression in murine TNBS-mediated intestinal injury and human NEC. Using fold-change cut-offs of ±1.5, we identified 4,440 differentially-expressed genes (DEGs) in murine TNBS-mediated injury and 1,377 in NEC. Murine TNBS-mediated injury and NEC produced similar changes in expression of orthologous genes (r = 0.611, P0.001), and also activated nearly-identical biological processes and pathways. Lipopolysaccharide was top predicted upstream regulator in both the murine and human datasets.Murine neonatal TNBS-mediated enterocolitis and human NEC activate nearly-identical biological processes, signaling pathways, and transcriptional networks.
- Subjects :
- Lipopolysaccharides
0301 basic medicine
Enteral administration
Article
Mice
03 medical and health sciences
0302 clinical medicine
Species Specificity
Enterocolitis, Necrotizing
030225 pediatrics
Gene expression
medicine
Animals
Humans
Gene Regulatory Networks
Intestinal Mucosa
Gene
Enterocolitis
Microarray analysis techniques
business.industry
Gene Expression Profiling
Transcriptional Networks
medicine.disease
digestive system diseases
3. Good health
Intestines
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
Animals, Newborn
Trinitrobenzenesulfonic Acid
Pediatrics, Perinatology and Child Health
Necrotizing enterocolitis
Cancer research
medicine.symptom
Signal transduction
business
Subjects
Details
- ISSN :
- 15300447 and 00313998
- Volume :
- 81
- Database :
- OpenAIRE
- Journal :
- Pediatric Research
- Accession number :
- edsair.doi.dedup.....4b4a4defdc60162ec86e6878dad1f9fc