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Decreased Tumorigenicity Correlates with Expression of Altered Cell Surface Carbohydrates in Lec9 CHO Cells

Authors :
J. Ripka
Seung-Il Shin
Pamela Stanley
Source :
Molecular and Cellular Biology. 6:1268-1275
Publication Year :
1986
Publisher :
Informa UK Limited, 1986.

Abstract

To investigate a role for surface carbohydrates in cellular malignancy, 15 different glycosylation-defective CHO cell mutants were examined for their tumorigenic and metastatic capacities after subcutaneous injection into nude mice. Most of the glycosylation mutants displayed similar or slightly decreased tumorigenicity compared with parental CHO cells. Neither parental CHO cells nor any of the mutants were observed to metastasize. However, independent isolates of one mutant type, Lec9, showed a dramatic reduction in tumor formation. The altered carbohydrates expressed at the surface of Lec9 cells appeared to be responsible for their loss of tumorigenicity, because revertants for lectin resistance were able to form tumors, and a double mutant (Lec9.Lec1) that expressed a Lec1 glycosylation phenotype also formed tumors. Finally, Lec9 cells were able to form tumors in gamma-irradiated nude mice, suggesting that recognition by an irradiation-sensitive host cell(s) was responsible for their reduced tumorigenicity in untreated nude mice.

Details

ISSN :
10985549
Volume :
6
Database :
OpenAIRE
Journal :
Molecular and Cellular Biology
Accession number :
edsair.doi.dedup.....4b45c52bffc81ef416698579108f548a
Full Text :
https://doi.org/10.1128/mcb.6.4.1268-1275.1986