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Modeling the Blood-Brain Barrier in a 3D triple co-culture microfluidic system
- Source :
- EMBC
- Publication Year :
- 2015
- Publisher :
- IEEE, 2015.
-
Abstract
- The need for a blood-brain barrier (BBB) model that accurately mimics the physiological characteristics of the in-vivo situation is well-recognized by researchers in academia and industry. However, there is currently no in-vitro model allowing studies of neuronal growth and/or function influenced by factors from the blood that cross through the BBB. Therefore, we established a 3D triple co-culture microfluidic system using human umbilical vein endothelial cells (HUVEC) together with primary rat astrocytes and neurons. Immunostaining confirmed the successful triple co-culture system consisting of an intact BBB with tight intercellular junctions in the endothelial monolayer. The BBB selective permeability was determined by a fluorescent-based assay using dextrans of different molecular weights. Finally, neuron functionality was demonstrated by calcium imaging.
- Subjects :
- Biology
Blood–brain barrier
Cell junction
Permeability
Umbilical vein
Calcium imaging
Lab-On-A-Chip Devices
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
Rats, Long-Evans
Neurons
Biological Transport
Dextrans
Coculture Techniques
Cell biology
Molecular Weight
medicine.anatomical_structure
nervous system
Blood-Brain Barrier
Permeability (electromagnetism)
Astrocytes
cardiovascular system
Neuron
Function (biology)
Immunostaining
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
- Accession number :
- edsair.doi.dedup.....4b30d58aa4c85aa5896ddd8bdb0b703e