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Methylene blue offers neuroprotection after intracerebral hemorrhage in rats through the PI3K/Akt/GSK3β signaling pathway
- Source :
- Journal of Cellular Physiology. 234:5304-5318
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Inflammation and apoptosis are two key factors contributing to secondary brain injury after intracerebral hemorrhage (ICH). In the present study, we explored the neuroprotective role of methylene blue (MB) in ICH rats and studied the potential mechanisms involved. Rats were subjected to local injection of collagenase IV in the striatum or sham surgery. We observed that MB treatment could exert a neuroprotective effect on ICH by promoting neurological scores, decreasing the brain water content, alleviating brain-blood barrier disruption, and improving the histological damages in the perihematomal areas. Furthermore, we demonstrated that the various mechanisms underlying MB's neuroprotective effects linked to inhibited apoptosis and inhibited neuroinflammation. In addition, wortmannin, a selective inhibitor of phosphoinositide 3-kinase (PI3K), could reverse the antiapoptotic and anti-inflammatory effects of MB, which suggested that the PI3K-Akt pathway played an important role. In conclusion, these data suggested that MB could inhibit apoptosis and ameliorate neuroinflammation after ICH, and its neuroprotective effects might be exerted via the activation of the PI3K/Akt/GSK3β pathway.
- Subjects :
- Male
0301 basic medicine
Physiology
Clinical Biochemistry
Anti-Inflammatory Agents
Apoptosis
Brain Edema
Inflammation
Pharmacology
Neuroprotection
Capillary Permeability
Rats, Sprague-Dawley
Wortmannin
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Animals
Protein kinase B
Neuroinflammation
PI3K/AKT/mTOR pathway
Cerebral Hemorrhage
Neurons
Glycogen Synthase Kinase 3 beta
Chemistry
Brain
Cell Biology
Methylene Blue
Disease Models, Animal
Neuroprotective Agents
030104 developmental biology
Neutrophil Infiltration
Blood-Brain Barrier
030220 oncology & carcinogenesis
Cytokines
Encephalitis
Microglia
Phosphatidylinositol 3-Kinase
medicine.symptom
Signal transduction
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 234
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....4b2b2c32ec472327a5a2272f2a955fa7